Microdevice for Direct DNA Purification

用于直接 DNA 纯化的微型装置

• 批准号: 7137457
• 负责人: James P Landers
• 金额: $ 23.86万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-19 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7137457
• 关键词: DNA; neoplasm /cancer; nucleic acid purification; polymers; tissues; university

DESCRIPTION (provided by applicant): With an ever-increasing interest in the molecular typing of cells from histologic tissue sections, the ability rapidly and efficiently extract DNA from the selected cells will be of paramount importance. The overall goal of this project is to develop a microchip-based sample preparation method for high efficiency, low-cost extraction of DNA from tissue samples - this microdevice will easily accommodate blood or other cells sources. The microdevices will be created using state-of-the-art microfabrication techniques coupled with fluidically controlled on-chip cell lysis and solid phase extraction chemistries. This project couples the industrial capabilities of HT Micro for facile fabrication of complex, high surface area microstructures, with surface modification chemistries developed at the University of Virginia that enable efficient and high capacity DNA extraction. The microdevices will be tested using a variety of sample varying in type and quality, and extraction efficiency will be determined using real-time PCR. As a demonstration of integration with current laser-capture microdissection instruments, the microdevice will be fabricated to directly accept the cap from the Arcturus Pixcell IIe which will contain the selected cells bound to an ethylene vinyl acetate polymer membrane on its bottom surface. The tissue samples will be collected and laser microdissected by our surgical pathology collaborator here at UVa - samples of normal and malignant cells will be analyzed. The final device will offer the rapid analysis, high extraction efficiency, and high-throughput advantages of microdevices and, in addition, is expected to offer higher capacity, and lower cost-per-device than current conventional or microchip techniques.

描述（由申请人提供）：随着对来自组织学组织切片的细胞的分子分型的兴趣不断增加，从所选细胞中快速有效地提取DNA的能力将是至关重要的。 该项目的总体目标是开发一种基于微芯片的样品制备方法，用于从组织样品中高效、低成本地提取DNA-这种微型设备将很容易容纳血液或其他细胞来源。 微器件将使用最先进的微制造技术，结合流体控制的芯片上细胞裂解和固相萃取化学来创建。 该项目将HT Micro的工业能力与弗吉尼亚大学开发的表面改性化学物质相结合，以轻松制造复杂的高表面积微结构，从而实现高效和高容量的DNA提取。 将使用各种类型和质量不同的样品对微型装置进行测试，并使用实时PCR确定提取效率。 作为与当前激光捕获显微切割仪器集成的证明，微型装置将被制造成直接接受来自Arcturus Pixcell IIe的帽，其将包含结合到其底部表面上的乙烯乙酸乙烯酯聚合物膜的选定细胞。 组织样本将被收集，并由我们在UVa的外科病理学合作者进行激光显微切割-正常和恶性细胞的样本将被分析。 最终的设备将提供快速分析，高提取效率和高通量的微型设备的优点，此外，预计将提供更高的容量，和更低的成本，每设备比目前的传统或微芯片技术。