Micro-Solid Phase Extraction of DNA on Microchips
微芯片上 DNA 的微固相提取
基本信息
- 批准号:6906510
- 负责人:
- 金额:$ 18.22万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-04-28 至 2006-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant):
Despite the current fad that the 'post-genome' era is coming to an end, it is likely that genetic analysis will continue to be a prolific field of exploration. Genetic analytical methods will be critical in fleshing out new mutations correlative of neoplastic growth, understanding the significance of single nucleotide polymorphisms, and developing better genotyping methods for microsatellite DNA. Accordingly, the purification of nucleic acid material from crude biological samples, a requisite protocol for successful amplification of target DNA sequences by the polymerase chain reaction (PCR), is still of vital importance. Development of a protocol for solid phase extraction (SPE) of DNA from biological samples (whole blood, cell culture, etc.) has been shown to be feasible in a microchip format during the previous funding period. This platform exploits the power of microchip technology for reducing sample volumes and increasing the speed of the extraction process, but optimization is needed to maximize the potential of this method. The additional benefit of the microchip platform is the ability to integrate additional processing steps. This will be investigated through the development of a microchip SPE-based DNA purification protocol that is directly and seamlessly mated with on-chip PCR, setting the stage for truly integrated genetic analysis on a microchip (a.k.a., a true lab-on-a-chip). A major part of the development work will also focus on integration of DNA quantitation methods directly on the microchip between the SPE and PCR steps. This will allow optimal use of the DNA extracted from the sample in the PCR amplification.
描述(由申请人提供):
尽管目前流行‘后基因组’时代即将结束,但基因分析很可能将继续是一个多产的探索领域。遗传分析方法对于充实与肿瘤生长相关的新突变、了解单核苷酸多态性的意义以及开发更好的微卫星DNA基因分型方法将是至关重要的。因此,从粗制的生物样品中提纯核酸物质,作为通过聚合酶链式反应(PCR)成功扩增目标DNA序列的必要程序,仍然是至关重要的。生物样品(全血、细胞培养等)中DNA固相萃取(SPE)方法的建立在上一个供资期间,微芯片格式已被证明是可行的。该平台利用了微芯片技术的力量,减少了样品体积,提高了提取过程的速度,但需要进行优化,以最大限度地发挥该方法的潜力。微芯片平台的另一个好处是能够集成额外的处理步骤。这将通过开发一种基于微芯片SPE的DNA纯化协议来进行研究,该协议直接与芯片上的PCR无缝配对,为在微芯片上进行真正的集成基因分析奠定基础(也就是真正的芯片实验室)。开发工作的一个主要部分还将侧重于将DNA定量方法直接整合到SPE和PCR步骤之间的微芯片上。这将使从样品中提取的DNA在聚合酶链式反应扩增中得到最佳使用。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
DNA extraction using a tetramethyl orthosilicate-grafted photopolymerized monolithic solid phase.
- DOI:10.1021/ac051796t
- 发表时间:2006-01
- 期刊:
- 影响因子:7.4
- 作者:Jian Wen;C. Guillo;J. Ferrance;J. Landers
- 通讯作者:Jian Wen;C. Guillo;J. Ferrance;J. Landers
2,3,5-Trimethyl-1,4-hydro-quinone.
2,3,5-三甲基-1,4-氢醌。
- DOI:10.1107/s1600536810000140
- 发表时间:2010
- 期刊:
- 影响因子:0
- 作者:Dai,Jun;Xie,Min-Hao;Liu,Ya-Ling;Zou,Pei;Wu,Hao
- 通讯作者:Wu,Hao
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
James P Landers其他文献
James P Landers的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('James P Landers', 18)}}的其他基金
2013 Physics and Chemistry of Microfluidics Gordon Research Conference & Gordon R
2013年微流控物理与化学戈登研究会议
- 批准号:
8517333 - 财政年份:2013
- 资助金额:
$ 18.22万 - 项目类别:
Micro-Solid Phase Extraction of DNA on Microchips
微芯片上 DNA 的微固相提取
- 批准号:
6741475 - 财政年份:2003
- 资助金额:
$ 18.22万 - 项目类别:
Micro-Solid Phase Extraction of DNA on Microchips
微芯片上 DNA 的微固相提取
- 批准号:
6618602 - 财政年份:2003
- 资助金额:
$ 18.22万 - 项目类别:
NON CONTACT INFRARED MEDIATED THERMOCYCLING FOR CHIP PCR
用于芯片 PCR 的非接触式红外介导热循环
- 批准号:
6181645 - 财政年份:1998
- 资助金额:
$ 18.22万 - 项目类别:
Non-contact microchip PCR based on infrared Heating
基于红外加热的非接触式微芯片PCR
- 批准号:
6679896 - 财政年份:1998
- 资助金额:
$ 18.22万 - 项目类别:
相似海外基金
Collaboration in Regulatory Systems Strengthening and Standardization Activities to Increase Global Access to Safe and Effective Biological Products.
加强监管系统和标准化活动方面的合作,以增加全球获得安全有效的生物产品的机会。
- 批准号:
10448926 - 财政年份:2021
- 资助金额:
$ 18.22万 - 项目类别:
Collaboration in Regulatory Systems Strengthening and Standardization Activities to Increase Global Access to Safe and Effective Biological Products.
加强监管系统和标准化活动方面的合作,以增加全球获得安全有效的生物产品的机会。
- 批准号:
10491861 - 财政年份:2021
- 资助金额:
$ 18.22万 - 项目类别:
Collaboration in Regulatory Systems Strengthening and Standardization Activities to Increase Global Access to Safe and Effective Biological Products.
加强监管系统和标准化活动方面的合作,以增加全球获得安全有效的生物产品的机会。
- 批准号:
10675535 - 财政年份:2021
- 资助金额:
$ 18.22万 - 项目类别:
EPSRC Centre for Doctoral Training in Bioprocess Engineering Leadership (Complex Biological Products Manufacture)
EPSRC 生物过程工程领导力博士培训中心(复杂生物制品制造)
- 批准号:
EP/S021868/1 - 财政年份:2019
- 资助金额:
$ 18.22万 - 项目类别:
Training Grant
Exploring interactions and benefits of novel microbial biological products in blueberry propagation
探索新型微生物生物制品在蓝莓繁殖中的相互作用和益处
- 批准号:
529840-2018 - 财政年份:2018
- 资助金额:
$ 18.22万 - 项目类别:
Applied Research and Development Grants - Level 1
GOALI: Collaborative Research: Industrial Implementation of Smart Biopolymers for Purification of Biological Products
目标:合作研究:用于生物制品纯化的智能生物聚合物的工业实施
- 批准号:
1403724 - 财政年份:2014
- 资助金额:
$ 18.22万 - 项目类别:
Standard Grant
GOALI: Collaborative Research: Industrial Implementation of Smart Biopolymers for Purification of Biological Products
目标:合作研究:用于生物制品纯化的智能生物聚合物的工业实施
- 批准号:
1403697 - 财政年份:2014
- 资助金额:
$ 18.22万 - 项目类别:
Standard Grant
Formulation and delivery approaches for water soluble biological products delivered through the skin focussing on L-Ascorbic Acid.
通过皮肤输送的水溶性生物产品的配方和输送方法,重点是 L-抗坏血酸。
- 批准号:
131690 - 财政年份:2014
- 资助金额:
$ 18.22万 - 项目类别:
Feasibility Studies
Formulation and stability of biological products for skin delivery focussing on Retinol.
以视黄醇为重点的皮肤输送生物制品的配方和稳定性。
- 批准号:
131338 - 财政年份:2013
- 资助金额:
$ 18.22万 - 项目类别:
Feasibility Studies
Demonstrate the effectiveness of antimicrobial protection of new types of Biological Products
展示新型生物制品抗菌保护的有效性
- 批准号:
429734-2011 - 财政年份:2012
- 资助金额:
$ 18.22万 - 项目类别:
Experience Awards (previously Industrial Undergraduate Student Research Awards)