MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

• 批准号: 7151714
• 负责人: Arion Kennedy
• 金额: $ 2.77万
• 依托单位: UNIVERSITY OF NORTH CAROLINA GREENSBORO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2009-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7151714
• 关键词: adipocytes; biological signal transduction; calcium ion; chemical conjugate; dairy products; dietary supplements; enzyme activity; enzyme inhibitors; essential fatty acids; glucose metabolism; glucose transport; human tissue; linoleate; lipid metabolism; lipid transport; meat; mitogen activated protein kinase; nuclear factor kappa beta; nutrition related tag; obesity; predoctoral investigator; protein kinase C; tissue /cell culture; weight loss; western blottings

DESCRIPTION (provided by applicant): The long-term goal of this research project is to identify novel dietary strategies for controlling human obesity, the most prevalent nutritional-related disease in America. The objective of this application is to identify upstream signals produced by an isomer of conjugated linoleic acid (CLA), fatty acids found in beef and dairy products and in dietary supplements for weight loss, that activate cell signals that lead to adipocyte delipidation. To complete this objective, the following specific aims will be carried out in cultures of human adipocytes: Aim #1) Determine the role of protein kinase C (PKC) in mediating CLA's activation of nuclear factor KB (NFKB) and extracellular signal-regulated kinase kinase (ERK)1/2 and delipidation; Aim #2) Identify the mechanism by which CLA increases [Ca2+]i, and its impact on NFKB and ERK1/2 signaling and delipidation; and Aim #3) Determine the extent to which CLA increases the activities of lipid metabolizing enzymes, generating signals that activate NFKB and ERK1/2 and promote delipidation. Data attained from this proposal are expected to provide critical insight for the development of therapeutic targets for the prevention and treatment of obesity and type II diabetes, and reduce health care costs related to obesity.

描述（由申请人提供）：本研究项目的长期目标是确定控制人类肥胖的新饮食策略，肥胖是美国最流行的营养相关疾病。本申请的目的是鉴定由共轭亚油酸（CLA）的异构体产生的上游信号，共轭亚油酸是在牛肉和乳制品以及用于减肥的膳食补充剂中发现的脂肪酸，其激活导致脂肪细胞脱脂的细胞信号。为了完成这一目标，将在人脂肪细胞培养物中进行以下具体目标：目标#1）确定蛋白激酶C（PKC）在介导CLA激活核因子KB（NF KB）和细胞外信号调节激酶激酶（ERK）1/2和去脂中的作用;目的#2）确定CLA增加[Ca 2 +]i的机制，及其对NF κ B和ERK 1/2信号传导和去脂的影响;和目的#3）确定CLA增加脂质代谢酶活性的程度，产生激活NF κ B和ERK 1/2并促进去脂的信号。从该提案中获得的数据预计将为开发预防和治疗肥胖和II型糖尿病的治疗靶点提供重要见解，并降低与肥胖相关的医疗保健成本。