Function, Localization, and Expression of Chl27

描述（由申请人提供）：我提出的研究旨在进一步表征叶绿素生物合成中涉及的有氧氧化环化酶反应。环化酶反应多年来一直为人所知，但其分子和遗传成分直到最近才为人所知。CHL27在拟南芥中被鉴定为该反应的一个组分，在莱茵衣藻中存在两个同源物CHL27A和CHL27B。我的研究将首先集中于阐明控制CN27A和CN27B亚细胞定位的机制。其次，由于CHL27在大麦病质体中的积累，我将描述其在叶绿体发育中的可能作用。第三，我将通过RNA干扰技术创建和表征CHL27B敲除突变体。最后，由于重组环化酶反应需要可溶性和膜组分，我将确定并进一步表征反应所需的附加组分。

DESCRIPTION (provided by applicant): My proposed research seeks to further characterize the aerobic oxidative cyclase reaction involved in chlorophyll biosynthesis. The cyclase reaction has been known for many years, however its molecular and genetic components remained unknown until recently. CHL27 was identified as being a component of the reaction in Arabidopsis thaliana, and two homologs, CHL27A and CHL27B, exist in Chlamydomonas reinhardtii. My research will first focus on elucidating the mechanisms that control the subcellular localization of CN27A and CN27B. Second, due to CHL27's accumulation in barley etioplasts, I will characterize its possible role in chloroplast development. Third, I will create and characterize a CHL27B knock out mutant through RNA interference techniques. And finally, because reconstituted cyclase reactions require both soluble and membrane components, I will identify and further characterize additional components required for the reaction.