Function, Localization, and Expression of Chl27

Chl27 的功能、定位和表达

• 批准号: 7064938
• 负责人: Michael D Allen
• 金额: $ 3.01万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7064938
• 关键词: Chlamydomonas; RNA interference; affinity chromatography; affinity labeling; angiosperms; biosynthesis; chlorophyll; chloroplasts; crosslink; fluorescence spectrometry; gene expression; gene mutation; oxygenases; plant genetics; plant proteins; predoctoral investigator; protein localization; protein purification; protozoal genetics; western blottings

DESCRIPTION (provided by applicant): My proposed research seeks to further characterize the aerobic oxidative cyclase reaction involved in chlorophyll biosynthesis. The cyclase reaction has been known for many years, however its molecular and genetic components remained unknown until recently. CHL27 was identified as being a component of the reaction in Arabidopsis thaliana, and two homologs, CHL27A and CHL27B, exist in Chlamydomonas reinhardtii. My research will first focus on elucidating the mechanisms that control the subcellular localization of CN27A and CN27B. Second, due to CHL27's accumulation in barley etioplasts, I will characterize its possible role in chloroplast development. Third, I will create and characterize a CHL27B knock out mutant through RNA interference techniques. And finally, because reconstituted cyclase reactions require both soluble and membrane components, I will identify and further characterize additional components required for the reaction.

描述（由申请人提供）：我提出的研究旨在进一步表征叶绿素生物合成中涉及的有氧氧化环化酶反应。环化酶反应已为人所知多年，但直到最近其分子和遗传成分仍然未知。 CHL27 被鉴定为拟南芥中反应的一个组分，并且两个同源物 CHL27A 和 CHL27B 存在于莱茵衣藻中。我的研究将首先集中于阐明控制 CN27A 和 CN27B 亚细胞定位的机制。其次，由于 CHL27 在大麦黄质体中积累，我将描述其在叶绿体发育中可能发挥的作用。第三，我将通过 RNA 干扰技术创建并表征 CHL27B 敲除突变体。最后，由于重构环化酶反应需要可溶性成分和膜成分，因此我将识别并进一步表征反应所需的其他成分。