Function, Localization, and Expression of Chl27

描述(由申请人提供)：我提议的研究旨在进一步表征参与叶绿素生物合成的有氧氧化循环酶反应。环酶反应早已为人所知，但其分子和遗传成分直到最近才为人所知。在拟南芥中，CHL27是该反应的一个组分，在莱茵衣藻中存在CHL27A和CHL27B两个同系物。我的研究将首先致力于阐明控制CN27A和CN27B亚细胞定位的机制。其次，由于CHL27的S在大麦黄体中的积累，我将对其在叶绿体发育中的可能作用进行表征。第三，我将通过RNA干扰技术创建并鉴定CHL27B基因敲除突变体。最后，由于重组环酶反应既需要可溶性成分，也需要膜成分，我将鉴定并进一步表征反应所需的其他成分。

DESCRIPTION (provided by applicant): My proposed research seeks to further characterize the aerobic oxidative cyclase reaction involved in chlorophyll biosynthesis. The cyclase reaction has been known for many years, however its molecular and genetic components remained unknown until recently. CHL27 was identified as being a component of the reaction in Arabidopsis thaliana, and two homologs, CHL27A and CHL27B, exist in Chlamydomonas reinhardtii. My research will first focus on elucidating the mechanisms that control the subcellular localization of CN27A and CN27B. Second, due to CHL27's accumulation in barley etioplasts, I will characterize its possible role in chloroplast development. Third, I will create and characterize a CHL27B knock out mutant through RNA interference techniques. And finally, because reconstituted cyclase reactions require both soluble and membrane components, I will identify and further characterize additional components required for the reaction.