CORE--MOUSE AND CELL LINE

核心——小鼠和细胞系

• 批准号: 7311603
• 负责人: STEFAN SOMLO
• 金额: $ 12.16万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7311603
• 关键词: animal breeding; animal colony; autosomal dominant trait; biomedical facility; cell line; gene mutation; genotype; laboratory mouse; polycystic kidney; tissue /cell preparation

The major goal of the Mouse and Cell Line Core is to provide Center investigators with common set of unique reagents and services to enable them to achieve the highest levels of insight into the problems in the PKD field. A secondary goal of the Core is to make these same reagents available to the research community at large. The Core reagents are and very comprehensive array of PKD-related animal models and well characterized mouse kidney cell lines with Pkd1 and Pkd2 mutations. The service provided by the Core is the expert consultation and collaboration to allow all Center investigators use these reagents to highest effect. The specific aims of the Core are: 1. To make available a comprehensive array of animal models relevant to the study of polycystic kidney and liver disease. 2. To characterize and make available specialized mouse-derived cell lines with fixed and inducible mutations in Pkd1 and Pkd2. 3. To perform the necessary mouse breeding and genotyping to obtain experimental animals. 4. To prepare tissues from experimental animals for imaging-based analysis. 5. To provide expert instruction to Center investigators in the design and study of new animal models. 6. To make mouse and cell line models available to the research community worldwide. The state-of-the-art in mouse models of human disease has moved from simple knockouts to complex combinations of conditional alleles with inducible transgenes. Issues of genetic background must always be borne in mind. New methods of BAG modification and transgenesis offer the ability to address very specific questions. All these approaches require expertise for optimal application. The Core and its PI will provide this service to all Center faculty as well as Center collaborators, thus facilitating and enhancing the respective projects.

鼠标和细胞系核心的主要目标是为中心调查人员提供一组常见的独特试剂和服务，以使其能够获得对PKD领域问题的最高水平。核心的次要目标是使这些试剂可用于整个研究界。核心试剂是与PKD相关的动物模型以及具有PKD1和PKD2突变的小鼠肾细胞系的非常全面的阵列。核心提供的服务是专家咨询和协作，允许所有中心研究人员使用这些试剂来最大程度地效果。 核心的具体目的是： 1。提供与多囊性肾脏和肝病研究有关的全面动物模型。 2。表征并使PKD1和PKD2中具有固定和可诱导突变的专业小鼠衍生细胞系。 3。执行必要的小鼠育种和基因分型以获得实验动物。 4。从实验动物制备基于成像的分析的组织。 5。为中心研究人员提供专家指导，以设计新动物模型的设计和研究。 6。使鼠标和细胞系模型可用于全球研究社区。 人类疾病小鼠模型中的最新技术已从简单的敲除变成了有条件等位基因与诱导型转基因的复杂组合。遗传背景问题必须始终牢记。袋装修改和转基因的新方法提供了解决非常具体问题的能力。所有这些方法都需要专业知识才能进行最佳应用。核心及其PI将为所有中心教师以及中心合作者提供这项服务，从而促进和增强各个项目。