A New Approach to the Production of Cultured Meat with Enhanced Texture

生产具有增强质感的培养肉的新方法

• 批准号: 2742212
• 金额: --
• 依托单位: University of Reading
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2742212
• 关键词: New; Approach; Production; Cultured; Meat

Future food production needs to meet a growing demand for food and changing diets, as the human population is predicted to reach 9 billion by 2050. Use of resources such as phosphorous and nitrogen are already exceeded sustainable limits, with agriculture placing large requirements on these resources. Further demands on the earth systems to increase food production will not be able to be supported. However current livestock production systems are major contributors to anthropogenic climate change and so, significant increases in production to meet growing demands will be unsustainable using current systems. Yet livestock products are nutrient dense and so cannot be discounted from diets without a suitable, healthy replacement. Therefore, transformative and disruptive production methods are needed to sustainably increase food production. Cultured meat is attracting great interest as a candidate for production of livestock products with reduced environmental impacts. This exciting project is to develop a new bioreactor method to prepare cultured meat. This technology produces aligned self-releasing tissue using a templating approach within the bioreactor. The approach has exceptional potential to produce aligned tissue for cultured meat tissue production with enhanced structural and textural properties. This is a collaborative project involving the University of Reading, Aberystwyth University and CASE partner Cellular Agriculture, a UK Small enterprise based in Llanelli, Wales.This cutting-edge project will investigate the production of aligned extracellular matrix within self-releasing tissue produced from myoblasts. This will use peptide amphiphile coatings on aligned templates in cell culture plates. The peptide amphiphiles stimulate cell adhesion and facilitate release of tissue using cell-expressed matrix metalloproteases (MMPs). The alignment template is a micropatterned Teflon substrate. This is prepared by a controlled rubbing process which leads to the production of microgrooves in the substrates. This templates the alignment of extracellular matrix produced by cells including collagen and other extracellular matrix proteins. The project involves the use of commercially available mouse myoblast cell lines for this initial work before extending the research to porcine cell lines. You will culture cells and examine the extracellular matrix produced using histochemical assays of extracellular matrix components, along with morphology and property studies and meat texture analysis, at the University of Reading. The project work at Aberystwyth will utilise the knowledge and expertise within the UK small company Cellular Agriculture's currently funded research projects at Aberystwyth and Bath on myoblast muscle cell sources and bioreactor designs to test the aligned extracellular matrix

未来的粮食生产需要满足不断增长的粮食需求和不断变化的饮食，因为预计到2050年人口将达到90亿。磷和氮等资源的使用已经超过可持续限度，农业对这些资源的需求很大。增加粮食产量对地球系统的进一步要求将无法得到支持。然而，目前的畜牧业生产系统是人为气候变化的主要原因，因此，利用现有系统大幅增加产量以满足日益增长的需求将是不可持续的。然而，畜产品营养丰富，因此如果没有合适的、健康的替代品，就不能从饮食中剔除。因此，需要变革和颠覆性的生产方法来可持续地增加粮食产量。作为减少环境影响的畜产品的候选产品，人造肉正引起人们的极大兴趣。这个令人兴奋的项目是开发一种新的生物反应器方法来制备培养肉。该技术利用生物反应器内的模板方法产生排列整齐的自我释放组织。该方法具有特殊的潜力，为培养肉组织生产具有增强的结构和质地特性的对齐组织。这是一个合作项目，涉及雷丁大学、阿伯里斯特威斯大学和CASE合作伙伴细胞农业，这是一家位于威尔士拉内利的英国小企业。这个前沿项目将研究在成肌细胞产生的自我释放组织中排列的细胞外基质的产生。这将在细胞培养板的对齐模板上使用肽两亲性涂层。肽两亲体通过细胞表达的基质金属蛋白酶（MMPs）刺激细胞粘附并促进组织释放。对准模板是微图案化的聚四氟乙烯基板。这是通过受控摩擦过程制备的，该过程导致在衬底上产生微槽。这种模板是由细胞产生的细胞外基质的排列，包括胶原蛋白和其他细胞外基质蛋白。该项目涉及在将研究扩展到猪细胞系之前，使用市售的小鼠成肌细胞系进行初步工作。您将在雷丁大学培养细胞，并使用细胞外基质成分的组织化学分析，以及形态学和特性研究以及肉质地分析来检查细胞外基质。Aberystwyth的项目工作将利用英国小公司Cellular Agriculture目前在Aberystwyth和Bath资助的成肌细胞肌肉细胞来源和生物反应器设计的研究项目中的知识和专业知识，以测试排列的细胞外基质