Genetic Dissection of Synaptic Transmission

突触传递的基因剖析

• 批准号: 7033081
• 负责人: Steve STOWERS
• 金额: $ 10.76万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7033081
• 关键词: Drosophilidae; arthropod genetics; cell morphology; fluorescence resonance energy transfer; gene deletion mutation; genetic screening; genetically modified animals; laboratory mouse; molecular cloning; monoclonal antibody; neural transmission; neurogenetics; neuromuscular junction; neuroregulation; phenotype; protein localization; protein structure function; single cell analysis; synaptic vesicles; transposon /insertion element

DESCRIPTION (provided by applicant): The long term objectives of this project are to understand the molecular and cellular physiology of the synapse as a prerequisite for comprehending more complex aspects of nervous system function and dysfunction. This research will likely have implications for many areas of neurobiology including synaptic plasticity, information processing, behavior, addiction, neurodegeneration, and psychiatric disease. The starting point of this research is an existing collection of 13 Drosophila mutants identified on the basis of a photoreceptor synaptic transmission phenotype. Specific aims include: 1) extending phenotypic characterization to include synapse ultrastructure and neuromuscular electrophysiology, 2) molecular cloning of the genes corresponding to the most interesting mutants, and 3) determining the molecular identity and cellular functions of the corresponding proteins. The candidate for this Research Career Award, Dr. R. Steven Stowers, has previously carried out molecular and genetic studies of synaptic transmission in the labs of Drs. Thomas Schwarz and Corey Goodman. Through this award, Dr. Stowers will take advantage of the electrophysiology and optical imaging expertise of his proposed mentor, Dr. Ehud Isacoff at UC Berkeley, to better understand the nature of the defects in his synaptic transmission mutants. The training Dr. Stowers will acquire in applying these biophysical techniques to neurobiological problems will complement his already strong background in genetics and molecular and cellular biology. In addition, the workshops, seminar series, and excellent neuroscience community at UC Berkeley provide an outstanding neuroscience training environment. Through expanding and diversifying his skill set to include electrophysiology and optical imaging, Dr. Stowers will develop into a formidable, well rounded neuroscientist ideally positioned to achieve both his immediate career goal of obtaining a faculty position at a research university and his long-term career goal of making significant contributions to neurobiology.

描述（由申请人提供）： 该项目的长期目标是了解突触的分子和细胞生理学，作为理解神经系统功能和功能障碍的更复杂方面的先决条件。这项研究可能会对神经生物学的许多领域产生影响，包括突触可塑性，信息处理，行为，成瘾，神经变性和精神疾病。本研究的出发点是现有的13个果蝇突变体的基础上确定的感光突触传递表型。具体目标包括：1）扩展表型表征以包括突触超微结构和神经肌肉电生理学，2）对应于最感兴趣的突变体的基因的分子克隆，和3）确定对应蛋白质的分子身份和细胞功能。这个研究职业奖的候选人，R。史蒂文·斯托尔斯此前曾在托马斯施瓦茨和科里·古德曼博士的实验室进行过突触传递的分子和遗传研究。通过这个奖项，Stowers博士将利用他的导师Ehud Isacoff博士在加州大学伯克利分校的电生理学和光学成像专业知识，更好地了解他的突触传递突变体缺陷的性质。Stowers博士将获得将这些生物物理技术应用于神经生物学问题的培训，这将补充他在遗传学、分子和细胞生物学方面已经很强的背景。此外，加州大学伯克利分校的研讨会，系列研讨会和优秀的神经科学社区提供了出色的神经科学培训环境。通过扩展和多样化他的技能，包括电生理学和光学成像，Stowers博士将发展成为一个强大的，全面的神经科学家，理想地实现他在研究型大学获得教职的直接职业目标和他对神经生物学做出重大贡献的长期职业目标。