Low Optical Coherence Interferometry for the Cochlea

耳蜗低光学相干干涉测量

• 批准号: 6998876
• 负责人: ALFRED L NUTTALL
• 金额: $ 19.25万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-17 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6998876
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; cochlea; ear hair cell; guinea pigs; interferometry; optical tomography; organ of Corti; vibration

DESCRIPTION (provided by applicant): The major goal in this proposal is to develop an instrument to measure the vibration of the organ of Corti in the living guinea pig. The instrument will use the principles of optical coherence tomography (OCT) and low optical coherence interferometry to visualize the basilar membrane and the internal structures of the organ and to measure their vibratory displacements induced by sound stimulation of the ear, respectively. The OCT interferometer will utilize light from two different super luminescent diodes coupled into a common optical fiber, allowing simultaneous measurement of the vibratory displacement of the basilar membrane and the reticular lamina components of the organ of Corti. The first two aims of the proposal are 1) to develop a dual source (two channels) OCT interferometer for measurements at a single location in the organ and 2) to develop a scanning OCT interferometer to measure organ of Corti motion in three dimensions. OCT interferometry will have the advantage of not requiring any reflective objects (such as mirrors or beads) to be placed on the ear tissues and will have high optical spatial resolution (particularly in the 'z' direction along the optical axis) allowing accurate magnitude and phase measurements of organ of Corti tissue vibration. The third aim is to use the OCT instrument to measure the three-dimensional vibration pattern of the basilar membrane and reticular lamina in the guinea pig. The data will give critical new insight into the micro mechanical motion of the organ of Corti and on the mechanism of the cochlear amplification process whereby outer hair cells are thought to be actively enhancing organ of Corti motion.

描述（由申请人提供）：本提案的主要目标是开发一种仪器来测量活体豚鼠柯蒂氏器官的振动。该仪器将利用光学相干断层扫描（OCT）和低光学相干干涉测量原理来可视化基底膜和器官的内部结构，并分别测量它们由耳朵的声音刺激引起的振动位移。 OCT 干涉仪将利用来自耦合到公共光纤的两个不同超发光二极管的光，从而可以同时测量基底膜和柯蒂氏器网状层组件的振动位移。该提案的前两个目标是 1) 开发双源（双通道）OCT 干涉仪，用于在器官的单个位置进行测量；2) 开发扫描 OCT 干涉仪，用于测量三维柯蒂运动器官。 OCT 干涉测量法的优点是不需要在耳组织上放置任何反射物体（例如镜子或珠子），并且具有高光学空间分辨率（特别是沿光轴的“z”方向），从而可以精确测量柯蒂氏器组织振动的幅度和相位。第三个目标是利用OCT仪器测量豚鼠基底膜和网状层的三维振动模式。这些数据将为柯蒂氏器的微机械运动和耳蜗放大过程的机制提供重要的新见解，据此外毛细胞被认为正在积极增强柯蒂氏器的运动。