COCHLEAR BLOOD FLOW AND NEUROPEPTIDES

偏头痛相关的内耳症状包括耳聋、耳鸣、听力波动、听力损失和噪声敏感性增加，这为连接基底动脉偏头痛和耳蜗病的病理生理机制提供了可能的神经基础的证据。最近，我们发现了一种以前未见报道的来自三叉神经节的耳蜗血管的感觉神经支配。我们已经证明，在正常和病理条件下(例如，在梅尼埃病的症状之一内淋巴积水的动物模型中)，这种感觉神经支配对耳蜗血流量(CBF)都有显著的影响。这项建议旨在进一步确定围绕椎弓和耳蜗血管系统的三叉神经-感觉网络的解剖学基础和机制。该建议提出的假设是，三叉神经感觉系统及其相关的神经肽系统是导致基底性偏头痛和耳蜗血管内稳态的重要因素。这项研究有三个具体目标。目的1.探讨基底动脉偏头痛的耳蜗性症状是否有生理学基础。阳性结果将证实基底性偏头痛和耳蜗炎的共同功能基础，基础可能是神经源性炎症。目的2.探讨香草素受体(VR1)和P物质(SP)是否共存于耳蜗血管、基底动脉及其相关分支。免疫细胞化学阳性结果将显示：(A)VR1和(B)SP共同标记的初级感觉神经元围绕基底动脉、小脑前下动脉(AICA)、螺旋蜗牛动脉(SMA)和桡动脉；(C)辣椒素可显著降低标记感觉纤维的密度。目的3.探讨内淋巴积水时三叉神经感觉神经元的血管调节功能障碍。在这项研究中，积极的结果将证明内淋巴积水导致刺激的三叉神经节引起的CBF改变减少。对该提议的研究将有助于阐明三叉神经感觉神经元在正常和病理条件下如何调节椎基底血管系统和耳蜗液平衡。

The migraine related inner ear symptoms for phonopobia , tinnitus, hearing fluctuation, hearing loss, and increased noise sensitivity provide evidence for a possible neurological substrate connecting basilar artery migraine and cochlear pathophysiological mechanisms. Recently we have identified a previously unreported sensory innervation of the cochlear blood vessels originating from the trigeminal ganglia. We have shown that this sensory innervation has a significant effect on cochlear blood flow (CBF) in both normal and pathological conditions (e.g., in the animal model of endolymphatic hydrops, one of the symptoms of Meniere's disease). This proposal seeks to further define the anatomical basis and mechanisms of the trigemino-sensory network around the vertebrovasilar and cochlear vascular system. The proposal offers the hypothesis that the trigemino-sensory system and its related neuropeptide system are important factors contributing to basilar migraine and vascular homeostasis of the cochlea. The study has three specific aims. Aim 1. To establish if there is a physiological basis for the cochlear symptoms in basilar artery migraine headache. Positive results will confirm a common functional basis for basilar migraine and cochlear symptoms, the basis could be neurogenic inflammation. Aim 2. To demonstrate if vanilloid receptor (VR1) and substance P (SP) are co-localized around cochlear blood vessels, the basilar artery and its related branches. Positive immunocytochemical results will demonstrate: (a) network of the VR1 and (b) SP co-labeled primary sensory neurons around the basilar artery; anterior inferior cerebellar artery (AICA), spiral modiolar artery (SMA) and radial artery; (c), Capsaicin will cause a significant reduction in the density of labeled sensory fibers. Aim 3. To determine the vasoregulatory disturbance of the trigemino-sensory neurons in endolymphatic hydrops. In this study positive results will demonstrate that endolymphatic hydrops causes a reduction in the stimulated trigeminal ganglion induced CBF change. The studies of the proposal will help clarify how trigemino-sensory neurons regulate the vertebro-basilar vascular system and cochlear fluid balance under normal and pathological conditions.