INFRASTRUCTURE 1: CORE RESEARCH LABORATORIES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。CBR/RCMI核心设施增强了塔斯基吉大学研究人员在显微镜/成像，流式细胞术，细胞培养和分子生物学，生物信息学和计算生物学方面的多学科研究。今年，核心设施向代表3个学院和9个科学学科的16个用户提供了服务和援助。这些研究者发表或提交了5篇同行评审论文，并提交了9篇论文或海报。成像设施继续为最多的研究人员提供服务，而细胞培养和分子生物学设施的使用“工时”最多。. Q成像CE数码相机通过C成像系统简单PCI软件操作最近被添加到光成像设施。这台相机大大提高了荧光显微镜的能力，设施，并应允许记录化学发光凝胶，而不使用射线照相胶片。Philips 400 TEM配备有科学仪器和应用（SIA）Pelter冷却CCD相机（320万像素）;使用SIA软件包（TIFF文件）捕获的图像进行处理和分析。通过购买几种最先进的仪器，分子生物学能力得到了显著提高。其中包括BIO-TEK SYNERGY HT酶标仪，可读取6至384孔板，并进行荧光、吸光度和发光测量。添加了Eppendorf主循环仪，更新了传统PCR。购买了Cepheid Smartcycler 2，可提供出色的实时PCR功能。该仪器采用4个光学通道;发射范围为510 nm至750 nm。这些通道允许同时检测四个“探针”。该仪器可实时显示生长曲线、温度曲线和熔解曲线，并进行定性、定量和生长曲线分析。生物信息学和计算生物学设施的目标是通过使用基于网络的基础设施，特别是在基因组学和蛋白质组学方面，增加教师对生物信息学和计算生物学研究的参与。我们已经添加了更新的软件，并继续我们的协会与亚拉巴马大学伯明翰基因组核心设施。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The CBR/RCMI Core Facility enhances multidisciplinary research of Tuskegee University investigators in Microscopy/Imaging, Flow Cytometry, Cell Culture and Molecular Biology, and Bioinformatics and Computational Biology. This year, the Core Facility has provided service and assistance to 16 users representing 3 academic colleges and 9 scientific disciplines. These investigators published or submitted 5 peer reviewed papers and presented 9 papers or posters. The Imaging Facility continues to offer service to the largest number of investigators while the Cell Culture and Molecular Biology facilities accumulated the most "man hours" of use. . A Q Imaging CE digital camera operating through C Imaging Systems Simple PCI software was recently added to the light imaging facility. This camera greatly enhances the fluorescent microscopy capabilities of the facility and should allow the recording of chemoluminescent gels without the use of radiographic film. The Philips 400 TEM was equipped with a Scientific Instruments & Applications (SIA) Pelter Cooled CCD Camera (3.2 megapixels); images captured using the SIA software package (TIFF files) are processed and analyzed. Molecular biology capabilities have been significantly enhanced through the purchase of several state of the art instruments. These include a BIO-TEK SYNERGY HT microplate reader, which reads 6-to-384 well plates and performs fluorescence, absorbance and luminescence measurements. An Eppendorf Mastercycler has been added updating conventional PCR. A Cepheid Smartcycler 2 has been purchased and provides outstanding Real-time PCR capabilities. The instrument employs 4 optical channels; emissions range from 510 nm to 750 nm. These channels permit simultaneous detection of four "probes". The instrument provides real-time display of growth curves, temperature profiles, and melting curves with qualitative, quantitative, and growth curve analysis. The goal of the Bioinformatics and Computational Biology Facility is to increase participation of faculty in bioinformatics and computational biology research through the use of web based infrastructure, especially in genomics and proteomics. We have added updated software from Accelrys and are continuing our association with the University of Alabama at Birmingham Genomics Core Facility.