INFRASTRUCTURE 1: CORE RESEARCH LABORATORIES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 塔斯基吉大学CBR/RCMI核心设施通过为研究人员提供利用显微镜/成像，流式细胞术，细胞培养和分子生物学以及生物信息学和计算生物学所需的仪器，设施和技术援助，促进多学科研究。核心设施已向代表3个学院和9个科学学科的用户提供了服务和援助。这些调查员在2007-2008年预算期发表或提交了十（10）篇同行评审论文，并提交了八（8）篇论文或海报。 大多数研究人员使用成像设备。 通过将Leica DM 5000 B（直立显微镜）与现有的Q Imaging CE数码相机（通过C Imaging Systems Simple PCI软件操作）连接，荧光显微镜得到了极大的增强。 该系统允许用多个荧光探针标记的样本的图像的可视化和数字捕获，以及将多个图像融合成单个照片。 此外，还获得了配备有细胞孵育室的Leica DM IRE 2（倒置荧光显微镜）。 该系统还配备了类似的数字摄影系统，并将允许活细胞实验在较长的时间段内可视化。 细胞培养和分子生物学实验室积累了最多的“工时”，因为它们为在卡弗研究实验室工作的研究人员（及其学生）提供现场设施。 生物信息学和计算生物学设施致力于通过使用基于网络的基础设施，特别是在基因组学和蛋白质组学方面，增加对生物信息学和计算生物学研究的参与。 为此，生物学系开设了两门生物信息学课程，生物学0368（本科）和0568（研究生）。 总之，在CBR/RCMI CORE I设施的5年支持期间，除了细胞培养和分子生物学设施（培养箱、离心机、冷冻箱等）的正常维护外，增加了几个提供更先进技术的新仪器。 这些包括：BIO-TEK SYNERGY HT酶标仪、Eppendorf Mastercycler、Cepheid Smartcycler 2和上述显微镜。 CORE I设施已向至少24名代表广泛科学研究领域的调查人员及其学生提供了服务。 这些研究者发表或提交了十七（17）篇同行评审论文，并提交了三十五（35）篇论文或海报。 总的来说，CORE I项目大大提高了塔斯基吉大学研究人员的研究能力和生产力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Tuskegee University CBR/RCMI Core Facility facilitates multidisciplinary research by providing investigators with the instrumentation, facilities and technical assistance necessary to utilize Microscopy/Imaging, Flow Cytometry, Cell Culture and Molecular Biology, and Bioinformatics and Computational Biology. The Core Facility has provided service and assistance to users representing 3 academic colleges and 9 scientific disciplines. These investigators published or submitted ten (10) peer reviewed papers and presented eight (8) papers or posters in the 2007-2008 budget period. The Imaging Facility is used by the most researchers. Fluorescence microscopy, has been greatly enhanced by the addition of a Leica DM 5000B (upright microscope) coupled to the existing Q Imaging CE digital camera (operating through C Imaging Systems Simple PCI software). This system allows the visualization and digital capturing of images of specimens tagged with multiple fluorescent probes and the fusion of the multiple images into a single photograph. Additionally, a Leica DM IRE2 (inverted fluorescent microscope) equipped with a cell incubation chamber has been acquired. This system is also equipped with a similar digital photography system and will allow the visualization of live cell experiments over extended time periods. The Cell Culture and Molecular Biology laboratories accumulate the most "man hours" of use as they provide on site facilities for the investigators (and their students) working in Carver Research Laboratories. The Bioinformatics and Computational Biology Facility strives to increase participation in bioinformatics and computational biology research through the use of web based infrastructure, especially in genomics and proteomics. Toward this end, the Department of Biology has initiated two courses in Bioinformatics, Biology 0368 (undergraduate) and 0568 (graduate). In summary, during the 5 years of support for the CBR/RCMI CORE I Facility, in addition to normal maintenance of the cell culture and molecular biology facilities (incubators, centrifuges, freezers, etc.), several new instruments providing more sophisticated technologies have been added. These include: BIO-TEK SYNERGY HT microplate reader, Eppendorf Mastercycler, Cepheid Smartcycler 2, and the microscopes described above. The CORE I Facility has provided services to at least 24 investigators and their students representing a broad spectrum of scientific research. These investigators published or submitted seventeen (17) peer reviewed papers and presented thirty-five (35) papers or posters. On the whole the CORE I project has significantly enhanced the research capabilities and productivity of the Tuskegee University research faculty.