The role of RNA modifications in the regulation of animal gene expression and germline development

RNA修饰在动物基因表达和种系发育调控中的作用

• 批准号: 2746742
• 金额: --
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2746742
• 关键词: role; RNA; modifications; regulation; animal

Epigenetic modifications of non-coding and messenger RNAs have been known for decades, and while some are now functionally well-defined and understood, the significance of several highly conserved modifications remain obscure. We are exploiting the manifold advantages of C. elegans as model system to investigate proteins involved in RNA methylation modifications that have essential roles in tissue development. These proteins have important but unknown roles during embryonic development in humans and other mammals, so the outcome of the research from this project will have direct impacts upon our understanding of human gene expression and development.The project will use a wide range of experimental tools to relate specific RNA methylation events to their roles in the gene expression changes underlying cell differentiation during development. The techniques used will include CRISPR-mediated-genome engineering, wide-field and confocal microscopy, long-read transcriptome analysis and liquid chromatography-tandem mass spectrometry proteomics. You will be joining a multidisciplinary research team (www.aberdeenwormlab.org) with a long-standing interest in RNA biochemistry, gene expression and model organism biology.

非编码RNA和信使RNA的表观遗传修饰已为人所知数十年，虽然其中一些修饰现在在功能上得到了明确的定义和理解，但一些高度保守的修饰的意义仍然模糊。我们正在利用秀丽隐杆线虫作为模型系统的多种优势来研究参与 RNA 甲基化修饰的蛋白质，这些蛋白质在组织发育中具有重要作用。这些蛋白质在人类和其他哺乳动物的胚胎发育过程中具有重要但未知的作用，因此该项目的研究结果将直接影响我们对人类基因表达和发育的理解。该项目将使用广泛的实验工具将特定的RNA甲基化事件与其在发育过程中细胞分化的基因表达变化中的作用联系起来。所使用的技术包括 CRISPR 介导的基因组工程、宽视野和共聚焦显微镜、长读长转录组分析和液相色谱-串联质谱蛋白质组学。您将加入一个对 RNA 生物化学、基因表达和模式生物生物学有着长期兴趣的多学科研究团队 (www.aberdeenwormlab.org)。