Understanding effects of formulations on ingredient interaction with tissue culture models of the oral epithelium

了解配方对成分与口腔上皮组织培养模型相互作用的影响

• 批准号: 2747248
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2747248
• 关键词: Understanding; effects; formulations; ingredient; interaction

This project will investigate the establishment and validation of tissue engineered oral mucosal equivalents as a high throughput screening tool for selected actives and excipients used in products to maintain and protect oral health.Specifically, we aim to(i) Investigate the barrier properties of oral mucosal equivalents (ii) Penetration and uptake of actives including chlorhexidine, CPC, CPC-zinc and zinc-isopropylmethyl phenol in oral equivalents and in porcine buccal tissue (iii) How the uptake of selected excipients commonly used in oral healthcare products influences the penetration of these actives (iv) If the nature of the excipients and quantities may be modified for optimal delivery of actives in oral equivalents (v)The capability of oral tissue equivalents to predict uptake of actives and excipients in porcine buccal tissue Objectives: 1. Establishment and characterisation of oral tissue equivalent models 2. Characterisation of active and excipient permeation in oral tissue equivalents using conventional analytical methods (GC, HPLC, NMR) flame absorption atomic spectroscopy as well as advanced techniques including confocal Raman spectroscopy. 3. Evaluate efficacy of oral tissue equivalents for engineering novel formulations for oral health conditions This project will establish new models of the oral epithelium for rapid development and screening of formulations for management of oral health and will be of considerable interest to academia and industry.

本项目将研究组织工程口腔粘膜等效物的建立和验证，作为维持和保护口腔健康产品中所用活性物质和赋形剂的高通量筛选工具。具体而言，我们的目标是（i）研究口腔粘膜等效物的屏障特性（ii）活性物质（包括氯己定，CPC，CPC-锌和锌-异丙基甲基苯酚在口腔等效物和猪颊组织中的含量（iii）口腔保健产品中常用的选定赋形剂的吸收如何影响这些活性物质的渗透（iv）如果可以改变赋形剂的性质和量以优化口服等效物中活性物质的递送（v）口腔组织等效物预测猪颊组织中活性物质和赋形剂摄取的能力目的：1.口腔组织等效模型的建立与表征2.使用常规分析方法（GC、HPLC、NMR）火焰吸收原子光谱法以及包括共聚焦拉曼光谱法在内的先进技术，表征活性物质和辅料在口腔组织等效物中的渗透。3.评估口腔组织等效物对口腔健康状况工程新配方的功效该项目将建立口腔上皮的新模型，用于快速开发和筛选口腔健康管理配方，并将引起学术界和工业界的极大兴趣。