Signaling in Tissue Injury and Repair

组织损伤和修复中的信号传导

• 批准号: 7077798
• 负责人: ROBERT F DIEGELMANN
• 金额: $ 11.6万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7077798
• 关键词: Signaling; Tissue; Injury; Repair

DESCRIPTION (provided by applicant): This is a revised renewal of the Training Program in Signaling in Tissue Injury & Repair. Trainees recruited into the program undergo an intense training in the basic science aspects of tissue repair research utilizing laboratories at Virginia Commonwealth University. It is intended that during the two year experience, trainees will be exposed to the following areas of tissue repair biology: 1) the biology and phenotypic regulation of mesenchymal cells in development and repair; 2) the protein and molecular biology of and regulation of expression of collagens, fibronectin and collagenases (matrix metalloproteinases); 3) the role of cytokines in the regulation of matrix expression in mesenchymal cells; 4) cytoskeletal proteins and signal transduction in mesenchymal cells; 5) the role of inflammation, nitric oxide and inflammatory cells such as mast cells, macrophages and platelets in tissue repair; 6) apoptosis and proto-oncogene expression; 7) the physical properties of connective tissues and bioengineering; 8) cellular and molecular signaling. Trainees will be encouraged to become familiar with the following techniques: 1) basic instrumentation including spectrophotometric and fluorescence techniques, HPLC, GLC, radioisotope counting and ultracentrifugation; 2) radioimmunoassay; 3) cell dispersion and isolation of subcellular organelles; 4) cell culture techniques; 5) isolation and purification and characterization of proteins (ion exchange chromatography, SDS/PAGE, Western blot analysis); 6) Northern and Southern blot analysis and PCR; 7) plasmid, phage and insert production, sub cloning and custom library production; and 8) small animal surgery. In addition to the laboratory training, each fellow is required to prepare and submit an individual NRSA as a learning experience to prepare them for their independent academic careers. Likewise, all fellows are required to submit proposals to other funding agencies such as the Plastic Surgery Educational Association, Jeffress Foundation and other sources in an effort to help support their research supply funds. Also, each fellow is taught how to prepare applications to the IRB and IACUC. Over the years this laboratory has trained approximately 30 fellows and many are productive scientists as academic physicians. This revised renewal proposal has been expanded and now includes a number of highly productive basic scientists with outstanding experience in the training of research fellows. Previously, many of these basic scientists were not engaged in the field of tissue repair and trauma, but now through this program they will become involved. In addition, this training program will seek applicants from Critical Care Medicine and Emergency Medicine as well as Surgery. The Program Director has made concerted efforts and will continue to strive to attract underrepresented minorities to apply to this unique training program.

描述（由申请人提供）：这是组织损伤和修复信号培训计划的修订更新。该项目招募的受训者在弗吉尼亚联邦大学的实验室进行组织修复研究的基础科学方面的强化训练。在为期两年的经验中，学员将接触到组织修复生物学的以下领域：1)间充质细胞在发育和修复中的生物学和表型调节；2)胶原蛋白、纤维连接蛋白、胶原酶（基质金属蛋白酶）的蛋白分子生物学及表达调控；3)细胞因子对间充质细胞基质表达的调控作用；4)细胞骨架蛋白与间充质细胞信号转导；5)炎症、一氧化氮和肥大细胞、巨噬细胞、血小板等炎症细胞在组织修复中的作用；6)细胞凋亡与原癌基因表达；7)结缔组织物理特性与生物工程；8)细胞和分子信号传导。鼓励学员熟悉以下技术：1)基本仪器，包括分光光度法和荧光技术，高效液相色谱法，GLC，放射性同位素计数和超离心；2)等;3)细胞分散和亚细胞器的分离；4)细胞培养技术；5)蛋白的分离纯化和鉴定（离子交换色谱、SDS/PAGE、Western blot分析）；6) Northern and Southern blot分析和PCR；7)质粒、噬菌体、插入体制作、亚克隆、定制文库制作；还有小动物手术。除了实验室培训外，每位研究员还需要准备并提交个人NRSA作为学习经验，为他们独立的学术生涯做好准备。同样，所有的研究员都需要向其他资助机构提交提案，如整形外科教育协会、杰弗里斯基金会和其他来源，以帮助支持他们的研究供应资金。此外，每个研究员都被教导如何准备申请IRB和IACUC。多年来，这个实验室培养了大约30名研究员，其中许多是作为学术医生的多产科学家。这一修订后的更新建议已得到扩大，现在包括一些在培养研究人员方面具有杰出经验的高产基础科学家。以前，这些基础科学家中的许多人不从事组织修复和创伤领域，但现在通过这个项目，他们将参与其中。此外，该培训计划将寻求来自重症医学和急诊医学以及外科的申请人。项目主任做出了协调一致的努力，并将继续努力吸引代表性不足的少数民族申请这一独特的培训项目。