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Signaling in Tissue Injury and Repair

组织损伤和修复中的信号传导

基本信息

批准号：
7668515
负责人：
ROBERT F DIEGELMANN
金额：
$ 15.46万
依托单位：
VIRGINIA COMMONWEALTH UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-07-01 至 2010-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7668515
关键词：
Signal Transduction Tissues injury and repair

项目摘要

DESCRIPTION (provided by applicant): This is a revised renewal of the Training Program in Signaling in Tissue Injury & Repair. Trainees recruited into the program undergo an intense training in the basic science aspects of tissue repair research utilizing laboratories at Virginia Commonwealth University. It is intended that during the two year experience, trainees will be exposed to the following areas of tissue repair biology: 1) the biology and phenotypic regulation of mesenchymal cells in development and repair; 2) the protein and molecular biology of and regulation of expression of collagens, fibronectin and collagenases (matrix metalloproteinases); 3) the role of cytokines in the regulation of matrix expression in mesenchymal cells; 4) cytoskeletal proteins and signal transduction in mesenchymal cells; 5) the role of inflammation, nitric oxide and inflammatory cells such as mast cells, macrophages and platelets in tissue repair; 6) apoptosis and proto-oncogene expression; 7) the physical properties of connective tissues and bioengineering; 8) cellular and molecular signaling. Trainees will be encouraged to become familiar with the following techniques: 1) basic instrumentation including spectrophotometric and fluorescence techniques, HPLC, GLC, radioisotope counting and ultracentrifugation; 2) radioimmunoassay; 3) cell dispersion and isolation of subcellular organelles; 4) cell culture techniques; 5) isolation and purification and characterization of proteins (ion exchange chromatography, SDS/PAGE, Western blot analysis); 6) Northern and Southern blot analysis and PCR; 7) plasmid, phage and insert production, sub cloning and custom library production; and 8) small animal surgery. In addition to the laboratory training, each fellow is required to prepare and submit an individual NRSA as a learning experience to prepare them for their independent academic careers. Likewise, all fellows are required to submit proposals to other funding agencies such as the Plastic Surgery Educational Association, Jeffress Foundation and other sources in an effort to help support their research supply funds. Also, each fellow is taught how to prepare applications to the IRB and IACUC. Over the years this laboratory has trained approximately 30 fellows and many are productive scientists as academic physicians. This revised renewal proposal has been expanded and now includes a number of highly productive basic scientists with outstanding experience in the training of research fellows. Previously, many of these basic scientists were not engaged in the field of tissue repair and trauma, but now through this program they will become involved. In addition, this training program will seek applicants from Critical Care Medicine and Emergency Medicine as well as Surgery. The Program Director has made concerted efforts and will continue to strive to attract underrepresented minorities to apply to this unique training program.

描述（由申请人提供）：这是组织损伤与修复信号传导培训计划的修订更新。该计划招募的学员利用弗吉尼亚联邦大学的实验室接受组织修复研究基础科学方面的强化培训。在两年的学习期间，学员将接触到组织修复生物学的以下领域：1）间充质细胞在发育和修复过程中的生物学和表型调控； 2) 胶原蛋白、纤连蛋白和胶原酶（基质金属蛋白酶）的蛋白质和分子生物学及其表达调节； 3）细胞因子在间充质细胞基质表达调节中的作用； 4）间充质细胞中的细胞骨架蛋白和信号转导； 5）炎症、一氧化氮和肥大细胞、巨噬细胞、血小板等炎性细胞在组织修复中的作用； 6) 细胞凋亡和原癌基因表达； 7）结缔组织物理特性及生物工程； 8) 细胞和分子信号传导。鼓励学员熟悉以下技术： 1）基本仪器，包括分光光度和荧光技术、HPLC、GLC、放射性同位素计数和超速离心； 2）放射免疫分析； 3）细胞分散和亚细胞器的分离； 4）细胞培养技术； 5) 蛋白质的分离纯化和表征（离子交换层析、SDS/PAGE、Western blot分析）； 6) Northern和Southern印迹分析和PCR； 7) 质粒、噬菌体和插入片段生产、亚克隆和定制文库生产； 8）小动物手术。除了实验室培训之外，每个研究员还需要准备并提交个人 NRSA 作为学习经历，为他们的独立学术生涯做好准备。同样，所有研究员都必须向其他资助机构（例如整形外科教育协会、杰弗里斯基金会和其他来源）提交提案，以帮助支持他们的研究供应资金。此外，每位研究员还学习如何准备向 IRB 和 IACUC 的申请。多年来，该实验室已培训了大约 30 名研究员，其中许多人都是多产的科学家和学术医生。修订后的更新提案得到了扩展，现在包括了一批高产的基础科学家，他们在培训研究员方面拥有杰出的经验。此前，这些基础科学家中的许多人并未从事组织修复和创伤领域，但现在通过这个项目，他们将涉足其中。此外，该培训计划还将寻求重症医学科、急诊医学科以及外科的申请者。项目主任齐心协力，并将继续努力吸引代表性不足的少数族裔申请这一独特的培训项目。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Protein synthesis inhibition as a potential strategy for metabolic down-regulation.

蛋白质合成抑制作为代谢下调的潜在策略。

DOI：
10.1016/j.resuscitation.2006.08.029
发表时间：
2007
期刊：
Resuscitation
影响因子：
6.5
作者：
Evans,MelissaC;Diegelmann,RobertF;Barbee,RWayne;Tiba,MHakam;Edwards,Eric;Sreedhar,Sue;Ward,KevinR
通讯作者：
Ward,KevinR

Coagulopathy and traumatic shock: characterizing hemostatic function during the critical period prior to fluid resuscitation.

凝血障碍和创伤性休克：表征液体复苏前关键时期的止血功能。

DOI：
10.1016/j.resuscitation.2009.09.017
发表时间：
2010
期刊：
Resuscitation
影响因子：
6.5
作者：
White,NathanJ;Martin,ErikaJ;Brophy,DonaldF;Ward,KevinR
通讯作者：
Ward,KevinR

Examining platelet-fibrin interactions during traumatic shock in a swine model using platelet contractile force and clot elastic modulus.

使用血小板收缩力和凝块弹性模量检查猪模型创伤性休克期间血小板-纤维蛋白的相互作用。

DOI：
10.1097/mbc.0b013e3283456c68
发表时间：
2011
期刊：
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis
影响因子：
0
作者：
White,NathanJ;Martin,ErikaJ;Brophy,DonaldF;Ward,KevinR
通讯作者：
Ward,KevinR

Coagulopathy during cardiac arrest and resuscitation in a swine model of electrically induced ventricular fibrillation.

电诱发心室颤动猪模型心脏骤停和复苏期间的凝血障碍。

DOI：
10.1016/j.resuscitation.2011.02.034
发表时间：
2011
期刊：
Resuscitation
影响因子：
6.5
作者：
White,NathanJ;Leong,BenjaminSieu-Hon;Brueckner,Jessica;Martin,ErikaJ;Brophy,DonaldF;Peberdy,MaryA;Ornato,Joseph;Ward,KevinR
通讯作者：
Ward,KevinR

Rapid closure of midgestational excisional wounds in a fetal mouse model is associated with altered transforming growth factor-beta isoform and receptor expression.