Signaling in Tissue Injury and Repair

描述（由申请人提供）：这是组织损伤与修复信号传导培训计划的修订版。被招募到该计划的受训人员在弗吉尼亚联邦大学的实验室进行组织修复研究的基础科学方面的密集培训。在为期两年的培训中，学员将接触到以下组织修复生物学领域：1）间充质细胞在发育和修复中的生物学和表型调控; 2）胶原蛋白、纤连蛋白和胶原酶的蛋白质和分子生物学以及表达调控（基质金属蛋白酶）; 3）细胞因子在间充质细胞中基质表达调节中的作用; 4）间充质细胞中的细胞骨架蛋白和信号转导; 5）炎症、一氧化氮和炎性细胞如肥大细胞、巨噬细胞和血小板在组织修复中的作用; 6）细胞凋亡和原癌基因表达; 7）结缔组织和生物工程的物理性质; 8）细胞和分子信号传导。本课程将鼓励学员熟悉以下技术：1）基本仪器，包括分光光度和荧光技术、HPLC、GLC、放射性同位素计数和超浓缩技术; 2）放射免疫分析; 3）细胞分散和亚细胞器分离; 4）细胞培养技术; 5）蛋白质的分离、纯化和表征（离子交换层析，SDS/PAGE，Western印迹分析）; 6）北方和南方印迹分析和PCR; 7）质粒、噬菌体和插入物产生，亚克隆和定制文库产生; 8）小动物外科。除了实验室培训外，每个研究员还必须准备并提交个人NRSA作为学习经验，为他们的独立学术生涯做好准备。同样，所有研究员都必须向其他资助机构提交建议，如整形外科教育协会、杰弗里斯基金会和其他来源，以帮助支持他们的研究供应基金。此外，每个研究员都被教导如何准备向IRB和IACUC的申请。多年来，该实验室已培训了约30名研究员，其中许多人是作为学术医生的多产科学家。这一修订后的续延建议已经扩大，现在包括一些在培训研究员方面具有杰出经验的高产基础科学家。以前，这些基础科学家中的许多人并不从事组织修复和创伤领域的研究，但现在通过这个项目，他们将参与进来。此外，该培训计划将寻求来自重症监护医学和急诊医学以及外科的申请人。方案主任已作出协调一致的努力，并将继续努力吸引代表性不足的少数群体申请参加这一独特的培训方案。

DESCRIPTION (provided by applicant): This is a revised renewal of the Training Program in Signaling in Tissue Injury & Repair. Trainees recruited into the program undergo an intense training in the basic science aspects of tissue repair research utilizing laboratories at Virginia Commonwealth University. It is intended that during the two year experience, trainees will be exposed to the following areas of tissue repair biology: 1) the biology and phenotypic regulation of mesenchymal cells in development and repair; 2) the protein and molecular biology of and regulation of expression of collagens, fibronectin and collagenases (matrix metalloproteinases); 3) the role of cytokines in the regulation of matrix expression in mesenchymal cells; 4) cytoskeletal proteins and signal transduction in mesenchymal cells; 5) the role of inflammation, nitric oxide and inflammatory cells such as mast cells, macrophages and platelets in tissue repair; 6) apoptosis and proto-oncogene expression; 7) the physical properties of connective tissues and bioengineering; 8) cellular and molecular signaling. Trainees will be encouraged to become familiar with the following techniques: 1) basic instrumentation including spectrophotometric and fluorescence techniques, HPLC, GLC, radioisotope counting and ultracentrifugation; 2) radioimmunoassay; 3) cell dispersion and isolation of subcellular organelles; 4) cell culture techniques; 5) isolation and purification and characterization of proteins (ion exchange chromatography, SDS/PAGE, Western blot analysis); 6) Northern and Southern blot analysis and PCR; 7) plasmid, phage and insert production, sub cloning and custom library production; and 8) small animal surgery. In addition to the laboratory training, each fellow is required to prepare and submit an individual NRSA as a learning experience to prepare them for their independent academic careers. Likewise, all fellows are required to submit proposals to other funding agencies such as the Plastic Surgery Educational Association, Jeffress Foundation and other sources in an effort to help support their research supply funds. Also, each fellow is taught how to prepare applications to the IRB and IACUC. Over the years this laboratory has trained approximately 30 fellows and many are productive scientists as academic physicians. This revised renewal proposal has been expanded and now includes a number of highly productive basic scientists with outstanding experience in the training of research fellows. Previously, many of these basic scientists were not engaged in the field of tissue repair and trauma, but now through this program they will become involved. In addition, this training program will seek applicants from Critical Care Medicine and Emergency Medicine as well as Surgery. The Program Director has made concerted efforts and will continue to strive to attract underrepresented minorities to apply to this unique training program.