Signaling in Tissue Injury and Repair

描述(由申请者提供)：这是组织损伤和修复信号方面的培训计划的修订更新。招募到该计划的受训人员利用弗吉尼亚联邦大学的实验室接受组织修复研究的基础科学方面的密集培训。在两年的经验中，学员将接触到下列组织修复生物学领域：1)间充质细胞在发育和修复中的生物学和表型调节；2)胶原蛋白、纤维连接蛋白和胶原酶(基质金属蛋白酶)的蛋白质和分子生物学及其表达的调节；3)细胞因子在调节间充质细胞基质表达中的作用；4)间充质细胞中的细胞骨架蛋白和信号转导；5)炎症、一氧化氮和炎性细胞如肥大细胞、巨噬细胞和血小板在组织修复中的作用；6)细胞凋亡和原癌基因表达；7)结缔组织的物理性质和生物工程；8)细胞和分子信号传递。学员将被鼓励熟悉以下技术：1)基本仪器，包括分光光度和荧光技术、高效液相、葡萄糖、放射性同位素计数和超速离心；2)放射免疫分析；3)细胞分散和亚细胞器的分离；4)细胞培养技术；5)蛋白质的分离、纯化和鉴定(离子交换层析、SDS/PAGE、Western印迹分析)；6)Northern和Southern印迹分析和聚合酶链式反应；7)质粒、噬菌体和插入体生产、亚克隆和定制文库生产；8)小动物外科手术。除了实验室培训外，每个研究员都需要准备并提交一份个人NRSA作为学习经验，为他们的独立学术生涯做好准备。同样，所有研究员都被要求向其他资助机构提交提案，如整形外科教育协会、杰弗里斯基金会和其他来源，以帮助支持他们的研究供应基金。此外，每个研究员还学习如何准备IRB和IACUC的申请。多年来，该实验室已经培训了大约30名研究员，其中许多人是多产的科学家，成为学术内科医生。这项修订后的延长建议已扩大，现在包括一些在培训研究人员方面具有杰出经验的高生产率的基础科学家。以前，这些基础科学家中的许多人并不从事组织修复和创伤领域，但现在通过这个项目，他们将参与其中。此外，该培训计划将招收重症监护医学和急诊医学以及外科的申请者。方案主任作出了协调一致的努力，并将继续努力吸引代表性不足的少数群体申请参加这一独特的培训方案。

DESCRIPTION (provided by applicant): This is a revised renewal of the Training Program in Signaling in Tissue Injury & Repair. Trainees recruited into the program undergo an intense training in the basic science aspects of tissue repair research utilizing laboratories at Virginia Commonwealth University. It is intended that during the two year experience, trainees will be exposed to the following areas of tissue repair biology: 1) the biology and phenotypic regulation of mesenchymal cells in development and repair; 2) the protein and molecular biology of and regulation of expression of collagens, fibronectin and collagenases (matrix metalloproteinases); 3) the role of cytokines in the regulation of matrix expression in mesenchymal cells; 4) cytoskeletal proteins and signal transduction in mesenchymal cells; 5) the role of inflammation, nitric oxide and inflammatory cells such as mast cells, macrophages and platelets in tissue repair; 6) apoptosis and proto-oncogene expression; 7) the physical properties of connective tissues and bioengineering; 8) cellular and molecular signaling. Trainees will be encouraged to become familiar with the following techniques: 1) basic instrumentation including spectrophotometric and fluorescence techniques, HPLC, GLC, radioisotope counting and ultracentrifugation; 2) radioimmunoassay; 3) cell dispersion and isolation of subcellular organelles; 4) cell culture techniques; 5) isolation and purification and characterization of proteins (ion exchange chromatography, SDS/PAGE, Western blot analysis); 6) Northern and Southern blot analysis and PCR; 7) plasmid, phage and insert production, sub cloning and custom library production; and 8) small animal surgery. In addition to the laboratory training, each fellow is required to prepare and submit an individual NRSA as a learning experience to prepare them for their independent academic careers. Likewise, all fellows are required to submit proposals to other funding agencies such as the Plastic Surgery Educational Association, Jeffress Foundation and other sources in an effort to help support their research supply funds. Also, each fellow is taught how to prepare applications to the IRB and IACUC. Over the years this laboratory has trained approximately 30 fellows and many are productive scientists as academic physicians. This revised renewal proposal has been expanded and now includes a number of highly productive basic scientists with outstanding experience in the training of research fellows. Previously, many of these basic scientists were not engaged in the field of tissue repair and trauma, but now through this program they will become involved. In addition, this training program will seek applicants from Critical Care Medicine and Emergency Medicine as well as Surgery. The Program Director has made concerted efforts and will continue to strive to attract underrepresented minorities to apply to this unique training program.