Crops & Soil - CRISPR-mediated precision gene editing in algae

CRISPR技术最近成为真核生物基因和基因组编辑的革命性工具。它为研究基因功能和调控基因表达开辟了新的途径，并具有巨大的潜力，以改善作物，包括藻类1。然而，我们对CRISPR介导的序列特异性DNA整合和替换的了解仍然有限，这限制了其全部潜力的开发。该项目将探索新的策略，以提高精确的基因工程在模式绿色衣藻莱茵衣藻和超越。我们的项目将提供一个框架，以发展核心研究技能以及优秀的跨学科研究培训，包括沟通和公众参与的技能。

The CRISPR technology has recently emerged as a revolutionary tool for gene and genome editing in eukaryotes. It opened new avenues to study gene function and modulate gene expression and has a great potential to improve crops including algae1. However, our knowledge is still limited on CRISPR-mediated sequence-specific DNA integration and replacement, which limits the exploitation of its full potential. This project will explore new strategies for improving precision gene engineering in the model green alga Chlamydomonas reinhardtii and beyond. Our project will provide the framework to develop core research skills as well as excellent cross-disciplinary research training, including skills in communication and public engagement.