Multidisciplinary Program in Female and Male Reproducti*

描述(由申请人提供)：马里兰大学医学院生殖研究专业合作中心计划(SCCPRR)的目标是对女性和男性生殖生物学进行创新的基础和临床研究，并应用这些研究获得的知识来提高对女性和男性生殖功能障碍和不孕不育的病因和治疗的了解。这项提案的主题重点是雌激素在调节灵长类动物胎儿卵巢和睾丸在宫内发育过程中的成熟以及在月经周期中子宫内膜的血管重构中所起的作用。在项目I(Gerald J.Pepe)中，将在狒狒身上评估子宫内雌激素在规划胎儿卵泡和卵母细胞发育过程中的调节作用，以及这对成年后生育能力的影响。在项目II(Eugene D.Albrecht)中，将评估子宫内雌激素在企划狒狒胎儿睾丸和流出小管/附睾发育过程中的调节作用，以及这对成年生育能力的影响。项目III(Robert D.Koos)提出了一项临床与基础研究相结合的研究，以确定雌激素和孕酮通过血管刺激因子和血管抑制因子的表达，在狒狒体内和体外共培养的人子宫内膜、血管内皮细胞和壁细胞中对子宫内膜血管生成和血管重塑的作用。我们将与马里兰大学SCCPRR、东弗吉尼亚医学院、匹兹堡大学SCCPRR和约翰霍普金斯大学SCCPRR的人类和非人类灵长类生殖生物学专家合作。每个研究项目都将在很大程度上依靠免疫细胞化学核心(Gloria E.Hoffman)来定位/表达调节因子和受体。这项SCCPRR中的科学努力的整合将通过关注雌激素在生殖系统发育成熟中的作用、将狒狒用作非人类灵长类动物模型以及合作研究的既定传统来实现。在狒狒灵长类动物模型中获得的发现有望转化为人类，以促进对人类生殖生物学的了解。

DESCRIPTION (provided by applicant): The objective of the Specialized Cooperative Centers Program in Reproduction Research (SCCPRR) at the University of Maryland School of Medicine is to conduct innovative basic and clinical research on female and male reproductive biology and to apply the knowledge gained from these studies to improve understanding of the etiology and treatment of reproductive dysfunction and infertility in women and men. The thematic focus of this proposal is the role that estrogen plays in regulating maturation of primate fetal ovaries and testes during intrauterine development and vascular remodeling of the uterine endometrium during the menstrual cycle. In Project I (Gerald J. Pepe), the regulatory role of estrogen in utero in programming fetal ovarian follicular and oocyte development and the impact this has on fertility in adulthood will be assessed in the baboon. In Project II (Eugene D. Albrecht), the regulatory role of estrogen in utero in programming baboon fetal testicular and excurrent ductule/epididymal development and the impact this has on fertility in adulthood will be assessed. A combined clinical-basic research study is proposed in Project III (Robert D. Koos), to determine the role of estrogen and progesterone, acting via expression of angiostimulatory and angioinhibitory factors, on endometrial angiogenesis and vascular remodeling in vivo in baboons and in vitro in cocultures of human endometrial and vascular endothelial and mural cells. We will collaborate with experts in human and nonhuman primate reproductive biology at the University of Maryland SCCPRR, Eastern Virginia Medical School, the University of Pittsburgh SCCPRR, and the Johns Hopkins University SCCPRR. Each of the research projects will draw heavily upon the immunocytochemistry Core (Gloria E. Hoffman) for the cellular localization/expression of regulatory factors and receptors. Integration of the scientific effort in this SCCPRR will be achieved through the thematic focus on estrogen's role in developmental maturation of the reproductive system, use of the baboon as a nonhuman primate model, and an established tradition of collaborative research. The discoveries obtained in the baboon primate model are expected to translate to the human to advance knowledge of human reproductive biology.