Core- CHIPS CORE

微阵列核心的总体目的是为中心内利用微阵列的特定项目提供支持，这些项目包括项目2(PI：Mary Fabry博士)将研究在补充精氨酸的饮食中小鼠的基因表达变化；项目3(Pi：Dhananjay Kaul)将研究黏附过程中的基因表达变化；以及项目4(Pi：Ronald Nagel)将研究在大鼠结肠系膜模型和转基因小鼠中参与镰状细胞血管闭塞的多效性和上位性基因。微阵列核心将为微阵列实验准备样本，执行微阵列杂交，并使用计算机软件分析数据，比较基因表达的相对变化。微阵列的打印和扫描是在AECOM微阵列设施进行的。为了确定人类群体中的多态性，CORE将对对照和镰状细胞患者DNA进行SNP分析。核心还负责微阵列结果的任何验证，包括半定量聚合酶链式反应或实时聚合酶链式反应方法的开发。该核心还将参与任何必要的方法开发，以提供用于微阵列实验的较小数量的RNA或杂交定制阵列。

The overall purpose of the Microarray Core is to provide support for specific projects within the center utilizing microarrays which include Project 2 (PI: Dr. Mary Fabry) who will be studying gene expression changes in mice on an arginine supplemented diet, Project 3 (PI: Dr. Dhananjay Kaul) who will be studying gene expression changes in adhesion processes, and Project 4 (PI: Dr. Ronald Nagel) who will be studying pleiotropic and epistatic genes involved in sickle cell vaso-occlusion in the rat mesocecum model and in transgenic mice. The Microarray Core will prepare samples for microarra experiments, perform the microarray hybridizations, and analyze the data using computer software that compares relative changes in gene expression. The printing and scanning of the microarrays are performed at the AECOM Microarray Facility. To define polymorphisms in the human population, the core will perform SNP analysis on control and sickle cell patient DNA. The core is also responsible for any validation of microarray results including semi-quantitative PCR or real time PCR methods development. The core will also be involved in any methods development necessary to labe smaller quantities of RNA for microarray experiments or to hybridize custom arrays.