Core- CHIPS CORE

Microarray Core的总体目的是为中心内使用微阵列的特定项目提供支持，其中包括项目2 （PI: Mary Fabry博士），他将研究精氨酸补充饮食中小鼠的基因表达变化，项目3 （PI: Dhananjay Kaul博士），他将研究粘附过程中的基因表达变化，以及项目4 （PI: Dhananjay Kaul博士）。罗纳德·内格尔博士)，他将在大鼠中皮层模型和转基因小鼠中研究镰状细胞血管闭塞的多效性和显性基因。Microarray Core将为微阵列实验准备样品，执行微阵列杂交，并使用计算机软件分析数据，比较基因表达的相对变化。微阵列的打印和扫描在AECOM微阵列设备上进行。为了确定人类群体中的多态性，核心将对对照和镰状细胞患者DNA进行SNP分析。核心还负责微阵列结果的任何验证，包括半定量PCR或实时PCR方法的开发。核心还将参与任何必要的方法开发标记小数量的RNA微阵列实验或杂交定制阵列。

The overall purpose of the Microarray Core is to provide support for specific projects within the center utilizing microarrays which include Project 2 (PI: Dr. Mary Fabry) who will be studying gene expression changes in mice on an arginine supplemented diet, Project 3 (PI: Dr. Dhananjay Kaul) who will be studying gene expression changes in adhesion processes, and Project 4 (PI: Dr. Ronald Nagel) who will be studying pleiotropic and epistatic genes involved in sickle cell vaso-occlusion in the rat mesocecum model and in transgenic mice. The Microarray Core will prepare samples for microarra experiments, perform the microarray hybridizations, and analyze the data using computer software that compares relative changes in gene expression. The printing and scanning of the microarrays are performed at the AECOM Microarray Facility. To define polymorphisms in the human population, the core will perform SNP analysis on control and sickle cell patient DNA. The core is also responsible for any validation of microarray results including semi-quantitative PCR or real time PCR methods development. The core will also be involved in any methods development necessary to labe smaller quantities of RNA for microarray experiments or to hybridize custom arrays.