Core--CHIP's

核心--CHIPs

• 批准号: 7406853
• 负责人: anne rybicki
• 金额: $ 17.48万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7406853
• 关键词: Arginine; Axon; Blood; Clinic; Computer software; Custom; DNA; Data Analyses; Epistatic Gene; Gene Expression; Genetic Polymorphism; Human; Hybridization Array; Institution; International; Label; Modeling; Mus; Patients; Polymerase Chain Reaction; Population; Printing; Purpose; RNA; Rattus; Relative (related person); Sampling; Scanning; Sickle Cell; Time; Transgenic Mice; Validation; adhesion process; dietary supplements; instrument; method development; research study

The overall purpose of the Microarray Core is to provide support for specific projects within the center utilizing microarrays which include Project 2 (PI: Dr. Mary Fabry) who will be studying gene expression changes in mice on an arginine supplemented diet, Project 3 (PI: Dr. Dhananjay Kaul) who will be studying gene expression changes in adhesion processes, and Project 4 (PI: Dr. Ronald Nagel) who will be studying pleiotropic and epistatic genes involved in sickle cell vaso-occlusion in the rat mesocecum model and in transgenic mice. The Microarray Core will prepare samples for microarra experiments, perform the microarray hybridizations, and analyze the data using computer software that compares relative changes in gene expression. The printing and scanning of the microarrays are performed at the AECOM Microarray Facility. To define polymorphisms in the human population, the core will perform SNP analysis on control and sickle cell patient DNA. The core is also responsible for any validation of microarray results including semi-quantitative PCR or real time PCR methods development. The core will also be involved in any methods development necessary to labe smaller quantities of RNA for microarray experiments or to hybridize custom arrays.

微阵列核心的总体目的是为中心内利用微阵列的特定项目提供支持，包括项目2（PI：玛丽法布里博士）谁将研究基因表达的变化，小鼠在精氨酸补充饮食，项目3（PI：Dhananjay Kaul博士）将研究粘附过程中的基因表达变化，（PI：罗纳德内格尔博士），他将在大鼠中盲肠模型和转基因小鼠中研究镰状细胞血管闭塞相关的多效性和上位性基因。 微阵列核心将为微阵列实验准备样本，进行微阵列杂交，并使用计算机软件分析数据，比较基因表达的相对变化。 微阵列的打印和扫描在AECOM微阵列设施中进行。 为了确定人类群体中的多态性，核心将对对照和镰状细胞患者DNA进行SNP分析。 该核心还负责微阵列结果的任何验证，包括半定量PCR或真实的时间PCR方法的开发。 该核心还将参与任何必要的方法开发，以标记用于微阵列实验或杂交定制阵列的少量RNA。