Developing DNA-based schistosomiasis blood diagnostics at the point of care in community settings

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In this project, we aim to develop disruptive technologies that enable highly sensitive and specific disease diagnosis, using DNA or RNA signatures, to be carried out on low-cost paper-based microfluidic assay formats, for use within low-resource communities, globally. The overall vision is to translate nucleic acid testing for both infectious and non-communicable diseases onto lateral flow devices, so that they can be used without centralised laboratory facilities. We have previously pioneered paper-based DNA testing, demonstrating its application in rural low-resource settings, and in this project our aim is, working with Global Access Diagnostics, to explore manufacturing solutions, including cassette design, reagent storage and power management, to enable an integrated assay flow. By way of an exemplar assay, we will develop blood based LAMP amplification with either optical or electrochemical outputs for test results to detect schistosomiasis. Such an assay has the potential to address the public health need for schistosomiasis diagnostics that are practical for use in low-resource settings yet can match the sensitivity and specificity of more complex assays. We will explore the efficacy of devices in field studies in Uganda and/or Tanzania, working with healthcare professionals and community leaders to inform the design and operation of the assay.