Developing DNA-Based Molecular Robots

开发基于 DNA 的分子机器人

• 批准号: 10630190
• 负责人: Reza Zadegan
• 金额: $ 14.4万
• 依托单位: NORTH CAROLINA AGRI & TECH ST UNIV
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630190
• 关键词: Advocate; Applications Grants; Biological Markers; Biopsy; Breast Cancer Cell; Breast Cancer Detection; Cellular biology; Collaborations; Communities; Consumption; Cost Allocation; DNA; DNA-Directed RNA Polymerase; Data; Detection; Diagnosis; Diagnostic; Diagnostic Procedure; Disease; Engineering; Equipment; Foundations; Future; Gene Expression; Genes; Genetic Transcription; Goals; Grant; Green Fluorescent Proteins; Image; Immunotherapy; In Vitro; Intelligence; Life; Link; Magnetic Resonance Imaging; Malignant Neoplasms; Medical; Medical Device; Medicine; Mentorship; Methods; MicroRNAs; Mission; Molecular; Molecular Biology; Molecular Machines; Monitor; Nanotechnology; Operative Surgical Procedures; Optics; Pathway interactions; Patients; Performance; Polymerase Chain Reaction; Positioning Attribute; Procedures; Property; Protein Biosynthesis; Proteins; Public Health; Qualifying; RNA; Radiation therapy; Reaction; Reaction Time; Research; Research Activity; Resource Allocation; Resources; Robot; Roentgen Rays; Scientist; Series; Signal Transduction; Specificity; Students; Testing; Therapeutic; Therapeutic Agents; Therapeutic procedure; Time; Training; Training Activity; Ultrasonography; United States National Institutes of Health; Work; X-Ray Computed Tomography; biophysical tools; cancer biomarkers; cancer cell; chemotherapy; clinical diagnostics; design; diagnostic platform; digital; disease diagnosis; feasibility research; gel electrophoresis; gene product; high volume manufacturing; improved; interest; malignant breast neoplasm; overexpression; prospective; prototype; reaction rate; repaired; response; self assembly; student training; synthetic biology; theranostics; therapeutic gene; tool; translational research program

SUMMARY The majority of medical diagnostic and therapeutic procedures depend on invasive methods and sophisticated tools. These medical practices are non-convenient for the patients, are expensive, are time- consuming, and require intensive use of equipment and professional resources. Therefore, there exists a critical need for medical devices and procedures that minimize the inconvenience, cost, and resource allocation. The PI's long-term goal is to develop intelligent molecular robots that detect diseases and treat them by providing appropriate responses that are timely, inexpensive, and convenient. PI's overall objectives for the proposed project are to construct a prototype of the envisioned robot, produce the preliminary data that will support submitting a targeted R01 grant at NIH, and establish a translational research program with support from his team. The PI's team will build a programmable DNA-based robot that aims at i) detecting specific RNA molecules and ii) generating amplified signals and select gene products. Leveraging PI's background, qualifications, and expertise that this proposal will advocate and the strong team he has assembled, he and his team are well-positioned to pursue this research. The proposed idea is likely to succeed because the PI's preliminary data demonstrates the project's feasibility. As a proof of concept, the team will design the robot to detect specific microRNA molecules that are known markers of breast cancer cells. The robot responds to the existence of the microRNAs by overexpressing the accompanying therapeutic gene. Modifying and altering domain properties of DNA-based molecular machines are inexpensive, fast, and efficient. Hence, the proposed work potentially has a broad reach – given the team can readily change the robot to enable detection of a host of biomolecules, including but not limited to various microRNAs. To achieve the goal of the proposed project, the PI will address the following three specific aims: • Aim 1: Design, construct, and characterize the robot's structural, optical, and functional properties, resulting in the construction of an efficient robot. • Aim 2: Develop target specificity and demonstrate the robot's diagnostic and therapeutic capabilities that enable both colorimetric detection and elimination of the target cancer cells. • Aim 3: Establish a vigorous translational research program and involve students in the research, provide mentorship and training for students to train the impactful workforce, scientists, and leaders of the future; in alignment with the mission of NIH. If successful, the proposed research will result in preliminary data that informs larger grant applications to develop molecular robots that perform diagnostic and therapeutics.

总结 大多数医学诊断和治疗程序依赖于侵入性方法， 复杂的工具这些医疗实践对患者来说是不方便的，昂贵的，耗时的- 消耗，并需要密集使用设备和专业资源。因此，存在一个 迫切需要医疗设备和程序，以尽量减少不便，成本和资源 配置中PI的长期目标是开发智能分子机器人， 通过提供及时、廉价和方便的适当反应， PI的总体目标为拟议项目是建立一个原型的设想机器人，生产 初步数据，将支持提交一个有针对性的R 01赠款在NIH，并建立一个翻译 在他的团队的支持下进行研究。PI的团队将建造一个基于DNA的可编程机器人 其目的是i）检测特定的RNA分子和ii）产生扩增的信号并选择基因 产品.利用PI的背景，资格和专业知识，本提案将倡导和 他组建了一个强大的团队，他和他的团队完全有能力进行这项研究。拟议 这个想法很可能会成功，因为PI的初步数据证明了该项目的可行性。以证明你 根据概念，该团队将设计机器人来检测已知标记的特定microRNA分子 乳腺癌细胞。机器人对microRNA的存在做出反应， 伴随治疗基因。修饰和改变基于DNA的分子的结构域性质 机器便宜、快速、高效。因此，拟议的工作可能具有广泛的影响力- 考虑到该团队可以很容易地改变机器人，使其能够检测许多生物分子，包括但不限于 仅限于各种microRNA。为达致建议项目的目的，主要研究者会行程以下事项 三个具体目标： 目标1：设计、构造和表征机器人的结构、光学和功能特性， 从而构造出高效的机器人。 目标2：开发目标特异性，展示机器人的诊断和治疗能力 其能够进行比色检测和消除靶癌细胞。 ·目标3：建立一个充满活力的转化研究计划，让学生参与研究， 为学生提供指导和培训，以培养有影响力的劳动力，科学家和领导者 未来;与NIH的使命保持一致。 如果成功，拟议的研究将产生初步数据，为更大的赠款申请提供信息， 开发出能进行诊断和治疗的分子机器人。