GENE EXPRESSIONS AND GENOMIC ANALYSIS CORE

基因表达和基因组分析核心

• 批准号: 7024383
• 负责人: SHERMAN Morton WEISSMAN
• 金额: $ 21.26万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7024383
• 关键词: antibody; biomedical facility; biotechnology; cell biology; chromatin immunoprecipitation; functional /structural genomics; gene expression; genetic regulation; hematology; hematopoiesis; hematopoietic stem cells; microarray technology; polymerase chain reaction; training

The Gene Expression and Genomic Analyses Core will provide access to and training in emerging technologies for molecular analysis of hematopoiesis and stem cell biology to investigators of the YCEMH. First, the Core will provide services in Gene Expression Microarray Technology including providing comprehensive, state-of-the-art Affymetrix and spotted glass slide microarray analyses, providing DNA microarray training and education via training seminars, one-on-one training sessions, and ongoing advice and guidance in the use of microarrays for a range of experiments including transcript analysis, ChlP-chip, and other applications, and by providing specialized services, evaluation and optimization of the application of microarray analysis to solve challenging hematopoiesis/stem cell biology research problems. One specific challenge is the evaluation of different microarray platforms for relative detection sensitivity and specificity for low abundance hematopoiesis-related genes. Second, the Core will provide services related to Genomics and Microarray Technology by providing novel microarrays, including genomic tiling arrays, the ABI 1700 DNA microarray, the human chromosome 22 array, and the human promoter array, to investigators of the YCEMH, by providing microarray training and education for use of these specialized microarrays, and by providing specialized services in array technology for the evaluation and optimization of genomic microarray technologies to researchers in hematopoiesis and stem cell biology. This includes access to an on-site microarray hybridization chamber and a dedicated computer workstation equipped for hematology/stem cell-related array data analyses. In addition, the Core will provide training, education, and ready access to relevant laboratory instrumentation for chromatin immunoprecipitation including hands-on training in ChIP and ChlP-chip experiments. Access to an ABI Real-Time iCycler PCR instrument will provide investigators necessary tools to perform quantitative ChlP-related assays. This Core will also maintain an up-to-date profile of antibodies useful in ChIP with emphasis on those validated by the Yale Proteomics Center for distribution to interested investigators. Providing the most advanced microarray and chromatin-based technologies to YCEMH investigators will facilitate discoveries in hematology/stem cell biology that advance our ability to understand, diagnose, treat, and prevent hematological diseases.

基因表达和基因组分析核心将为YCEMH的研究人员提供造血和干细胞生物学分子分析新兴技术的访问和培训。首先，核心将提供基因表达微阵列技术方面的服务，包括提供全面的、最先进的Affyoung和斑点载玻片微阵列分析，通过培训研讨会、一对一培训课程提供DNA微阵列培训和教育，以及在一系列实验中使用微阵列的持续建议和指导，包括转录本分析、ChIP芯片和其他应用，并通过提供专业服务，评估和优化应用程序， 微阵列分析解决具有挑战性的造血/干细胞生物学研究问题。一个具体的挑战是评估不同微阵列平台对低丰度造血相关基因的相对检测灵敏度和特异性。第二，核心将提供与基因组学和微阵列技术相关的服务，通过向YCEMH的研究人员提供新型微阵列，包括基因组平铺阵列、ABI 1700 DNA微阵列、人类22号染色体阵列和人类启动子阵列，通过提供微阵列培训和教育来使用这些专门的微阵列，并提供阵列技术方面的专业服务， 基因组微阵列技术给造血和干细胞生物学的研究人员。这包括使用现场微阵列杂交室和专用计算机工作站，该工作站配备用于血液学/干细胞相关阵列数据分析。此外，核心将提供培训，教育，并随时获得染色质免疫沉淀的相关实验室仪器，包括ChIP和ChIP芯片实验的实践培训。ABI实时iCycler PCR仪器的使用将为研究者提供必要的工具来进行ChIP相关的定量测定。该核心还将保持对ChIP有用的抗体的最新概况，重点是耶鲁蛋白质组学中心验证的抗体，以分发给感兴趣的研究人员。为YCEMH研究人员提供最先进的微阵列和基于染色质的技术将促进血液学/干细胞生物学的发现，提高我们理解，诊断，治疗和预防血液疾病的能力。