GENE EXPRESSIONS AND GENOMIC ANALYSIS CORE

基因表达和基因组分析核心将为YCEMH的研究人员提供对造血和干细胞生物学的分子分析的新兴技术的访问和培训。首先，核心将提供基因表达微阵列技术的服务，包括提供全面的、最先进的Affymetrix和斑点玻璃载玻片微阵列分析，通过培训研讨会、一对一培训课程提供DNA微阵列培训和教育，以及在使用微阵列进行一系列实验(包括转录分析、ChlP芯片和其他应用)方面持续提供建议和指导，以及通过提供专门的服务、评估和应用程序优化 基因芯片分析，以解决具有挑战性的造血/干细胞生物学研究问题。一个特殊的挑战是评估不同的微阵列平台对低丰度造血相关基因的相对检测灵敏度和特异性。第二，核心将提供与基因组学和微阵列技术相关的服务，为青年会的研究人员提供新型微阵列，包括基因组平铺阵列、ABI 1700 DNA微阵列、人类22号染色体阵列和人类启动子阵列，提供使用这些专门微阵列的微阵列培训和教育，并提供阵列技术方面的专业服务，以进行评估和优化 向造血学和干细胞生物学的研究人员介绍基因组微阵列技术。这包括进入现场微阵列杂交室和配备用于血液学/干细胞相关阵列数据分析的专用计算机工作站。此外，核心将提供培训、教育，并随时获得染色质免疫沉淀的相关实验室仪器，包括芯片和ChlP芯片实验的动手培训。使用ABI Real-Time iCycler PCR仪器将为研究人员提供必要的工具来进行与ChlP相关的定量分析。该核心还将保持芯片中有用的抗体的最新概况，重点是那些由耶鲁蛋白质组学中心验证的抗体，以便分发给感兴趣的研究人员。向YCEMH研究人员提供最先进的微阵列和染色质技术将促进血液学/干细胞生物学的发现，提高我们了解、诊断、治疗和预防血液病的能力。

The Gene Expression and Genomic Analyses Core will provide access to and training in emerging technologies for molecular analysis of hematopoiesis and stem cell biology to investigators of the YCEMH. First, the Core will provide services in Gene Expression Microarray Technology including providing comprehensive, state-of-the-art Affymetrix and spotted glass slide microarray analyses, providing DNA microarray training and education via training seminars, one-on-one training sessions, and ongoing advice and guidance in the use of microarrays for a range of experiments including transcript analysis, ChlP-chip, and other applications, and by providing specialized services, evaluation and optimization of the application of microarray analysis to solve challenging hematopoiesis/stem cell biology research problems. One specific challenge is the evaluation of different microarray platforms for relative detection sensitivity and specificity for low abundance hematopoiesis-related genes. Second, the Core will provide services related to Genomics and Microarray Technology by providing novel microarrays, including genomic tiling arrays, the ABI 1700 DNA microarray, the human chromosome 22 array, and the human promoter array, to investigators of the YCEMH, by providing microarray training and education for use of these specialized microarrays, and by providing specialized services in array technology for the evaluation and optimization of genomic microarray technologies to researchers in hematopoiesis and stem cell biology. This includes access to an on-site microarray hybridization chamber and a dedicated computer workstation equipped for hematology/stem cell-related array data analyses. In addition, the Core will provide training, education, and ready access to relevant laboratory instrumentation for chromatin immunoprecipitation including hands-on training in ChIP and ChlP-chip experiments. Access to an ABI Real-Time iCycler PCR instrument will provide investigators necessary tools to perform quantitative ChlP-related assays. This Core will also maintain an up-to-date profile of antibodies useful in ChIP with emphasis on those validated by the Yale Proteomics Center for distribution to interested investigators. Providing the most advanced microarray and chromatin-based technologies to YCEMH investigators will facilitate discoveries in hematology/stem cell biology that advance our ability to understand, diagnose, treat, and prevent hematological diseases.