Cytokines and lineage choice in hematopoietic precursors
造血前体细胞的细胞因子和谱系选择
基本信息
- 批准号:8613792
- 负责人:
- 金额:$ 28.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-09-15 至 2016-06-30
- 项目状态:已结题
- 来源:
- 关键词:AccountingAdultAftercareAliquotBlood CellsCD34 geneCD34+ precursorCSF3 geneCell Culture TechniquesCell LineageCell TherapyCell physiologyCellsDNA MethylationDataDevelopmentEmerging TechnologiesErythroidErythropoietinExposure toGene ExpressionGene Expression ProfileGene SilencingGenesGenetic TranscriptionGenomicsGlycophorin AGoalsGrantGranulocyte-Macrophage Colony-Stimulating FactorHarvestHematopoieticHematopoietic stem cellsHeterogeneityHourHumanIndividualInstructionLabelLengthMeasurementMeasuresMethylationMolecularMyelogenousNatureOligonucleotidesPatternPopulationProductionRepressionRoleSiteSorting - Cell MovementSpecificityStimulusTFRC geneTestingThrombopoietinTimeTranscriptUse of New Techniquescell typecytokineinsightmRNA Expressionmethod developmentperipheral bloodprecursor cellpublic health relevanceresearch studyresponsesingle cell analysistelomeretranscriptome sequencing
项目摘要
Abstract: Human CD34+ hematopoietic precursor cells can readily be obtained from the peripheral blood of
adults. Treatment of these cells with various cytokine cocktails can induce efficient production of cells
differentiated along erythroid, megakaryocytic or myeloid lines. We find that different cytokine cocktails induce
rapid and extensive patterns of change in gene expression that are different for different cytokine cocktails.
The prominence of these changes suggests that they occur in a large fraction of the CD34+ cells and may
modulate the patterns of differentiation of individual cells. The recent development of methods that permit
global measurements of mRNA expression, and DNA methylation in single cells make it possible to investigate
the role of cytokines in lineage promotion at a level of specificity hitherto unavailable. In the present application
we propose to use a combination of single cell genomic scale analyses, and cell tagging and labeling
approaches to establish if the same cell can respond differently to different cytokine treatments, and to
investigate whether these different responses result in differentiation along alternate lineages. The ultimate
goal is to determine the nature of early transcriptional responses to lineage specific cytokines and whether
these are instructional as well as permissive for lineage specification.
摘要:人CD34+造血前体细胞可以很容易地从外周血中获得
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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SHERMAN Morton WEISSMAN其他文献
SHERMAN Morton WEISSMAN的其他文献
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{{ truncateString('SHERMAN Morton WEISSMAN', 18)}}的其他基金
Cytokines and lineage choice in hematopoietic precursors
造血前体细胞的细胞因子和谱系选择
- 批准号:
8735141 - 财政年份:2013
- 资助金额:
$ 28.97万 - 项目类别:
PREDICTIVE AND THERAPEUTIC UTILITIES OF EPIGENETIC CHANGES IN CHROMATIN IN MELANO
黑色素染色质表观遗传变化的预测和治疗用途
- 批准号:
7147298 - 财政年份:2006
- 资助金额:
$ 28.97万 - 项目类别:
DNA methylation in normal versus malignant melanocytes
正常黑素细胞与恶性黑素细胞中的 DNA 甲基化
- 批准号:
6952686 - 财政年份:2004
- 资助金额:
$ 28.97万 - 项目类别:
Global Analysis of Chromatin during Lineage Development
谱系发育过程中染色质的整体分析
- 批准号:
7881180 - 财政年份:2004
- 资助金额:
$ 28.97万 - 项目类别:
Global Analysis of Chromatin during Lineage Development
谱系发育过程中染色质的整体分析
- 批准号:
7455839 - 财政年份:2004
- 资助金额:
$ 28.97万 - 项目类别:
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