Interaction between islets and implantable immunoisolation polyurethane membranes

胰岛和植入式免疫隔离聚氨酯膜之间的相互作用

• 批准号: 7107074
• 负责人: STANLEY DALE HARPSTEAD
• 金额: $ 10万
• 依托单位: RST IMPLANTED CELL TECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-30 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7107074
• 关键词: artificial membranes; biomaterial compatibility; biomaterial evaluation; biotechnology; cell adhesion; cell cell interaction; cell population study; hydropathy; laboratory rat; laminin; medical implant science; newborn animals; pancreas; pancreatic islet function; pancreatic islets; polyurethanes; surface coating; technology /technique development; tissue /cell culture; tissue engineering; total artificial organ

DESCRIPTION (provided by applicant): The development of bio-artificial organs (sequestered cell cultures, implanted within an isolating membrane) is complicated by many dynamic and interrelated variables. 1 major variable is the relationship between the cell cultures contained in the membrane and the membrane, which will have a direct impact on the ability of the sequestered chamber to provide the desired therapeutic effects. This Phase 1 SBIR grant application will result in the development of methods to be used for mechanically segregating pancreatic islets clusters or B cells within a membrane chamber. The first specific aim of this grant is to test the feasibility of mechanically separating islets harvested from neonatal rats within an isolating membrane during in-vitro cell culture experiments. The second aim of this grant is to determine the adhesion properties between B cells in culture with a laminin coated polyurethane membrane. The third aim of this grant is to compare the insulin production from islet clusters that have been segregated by mechanical means (specific aim 1) to insulin production from beta cells that have been individually stabilized by adhesion to the laminin coated membrane (specific aim 2). The results from this Phase I project will directly impact the specific design criteria of a clinically useful cell chamber device. The information gained by studying the interactions between islets,B-cells, the polyurethane membrane and surface modification using laminin will provide essential insight into the structure, dimensions and capacity of a larger medical device used for the treatment of type-1 diabetes. It is our intention to pursue the specific device development in concert with a Phase II grant application in conjunction with other corporate collaborations.

描述(由申请人提供)：生物人造器官的发展(隔离细胞培养，植入隔离膜内)是由许多动态和相互关联的变量而复杂的。1主要变量是膜中包含的细胞培养物与膜之间的关系，这将直接影响隔离室提供所需治疗效果的能力。这一阶段的SBIR赠款申请将导致开发用于在膜室中机械分离胰岛簇或B细胞的方法。这笔赠款的第一个具体目标是测试在体外细胞培养实验中，在隔离膜内机械分离新生大鼠胰岛的可行性。这项资助的第二个目的是确定层粘连蛋白涂层聚氨酯膜培养中B细胞之间的粘附性。这笔赠款的第三个目的是比较通过机械手段分离的胰岛簇产生的胰岛素(特定目标1)和通过黏附到层粘连蛋白涂层膜上单独稳定的β细胞产生的胰岛素(特定目标2)。这个第一阶段项目的结果将直接影响临床上有用的细胞室设备的具体设计标准。通过研究胰岛、B细胞、聚氨酯膜和使用层粘连蛋白的表面修饰之间的相互作用获得的信息将为用于治疗1型糖尿病的更大医疗设备的结构、尺寸和容量提供必要的洞察。我们的意图是与其他公司合作，在第二阶段拨款申请的同时，继续进行特定设备的开发。