Microarray chips for rapid detection of high affinity nucleic acid sequences

用于快速检测高亲和力核酸序列的微阵列芯片

• 批准号: 7159145
• 负责人: Mark Patrick McPike
• 金额: $ 11.25万
• 依托单位: APTAMATRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7159145
• 关键词: biotechnology; drug discovery /isolation; genetic library; high throughput technology; lysozyme; method development; microarray technology; nucleic acid quantitation /detection; nucleic acid sequence; nucleocapsid; oligonucleotides; protein binding; virus protein

DESCRIPTION (provided by applicant): Project Summary/Abstract: This phase I project aims at demonstrating a method to rapidly discover short sequences that bind tightly to protein targets. Our plan in phase I is limited to proof of principle by screening against fluorescently tagged proteins, which will facilitate analysis. In phase II, label-free detection methods of analysis will be pursued in an effort to generalize the screening method. Oligonucleotides discovered in these screens can be engineered into sensors having exquisite sensitivity and selectivity for diagnostics and drug discovery applications.

描述（由申请人提供）：项目摘要/摘要：本阶段I项目旨在展示一种快速发现与蛋白质靶点紧密结合的短序列的方法。我们在第一阶段的计划仅限于通过筛选荧光标记蛋白质来证明原理，这将有助于分析。在第二阶段，将采用无标记检测分析方法，以推广筛选方法。在这些筛选中发现的寡核苷酸可以被设计成具有用于诊断和药物发现应用的灵敏度和选择性的传感器。