Vascular Protective Role of Endothelin B Receptors

内皮素 B 受体的血管保护作用

• 批准号: 7125509
• 负责人: Raouf A Khalil
• 金额: $ 34.18万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7125509
• 关键词: blood pressure; calcium flux; confocal scanning microscopy; cyclic AMP; cyclic GMP; dietary sodium; digital imaging; endothelin; enzyme activity; hormone receptor; immunofluorescence technique; inhibitor /antagonist; isozymes; kidney; laboratory rat; nitric oxide; nutrition related tag; prostacyclins; protein kinase C; protein structure function; urinalysis; vascular resistance; vascular smooth muscle; vasoconstriction; western blottings

DESCRIPTION (provided by applicant): High salt diet is often associated with increased vascular resistance and arterial pressure in salt-sensitive individuals. In normal individuals, however, high salt diet does not increase the arterial pressure significantly, suggesting possible vascular protective mechanisms. Endothelin-1 (ET-1) activates ETA and ETa receptors. Although the role of ETA receptors in vascular contraction and hypertension has been studied extensively, the importance of ETa receptors in modulating the vascular function and arterial pressure particularly during high salt diet is less clear. Preliminary data suggest that ET-1 production is enhanced during high sodium intake. Data also suggest that chronic blockade of ETa receptors results in a salt-sensitive form of hypertension; however, the vascular and cellular mechanisms involved are unclear. The overall objective of this proposal is to test the hypothesis that normally during high salt diet an increase in ET-1 production and enhancement of its ETA-mediated vascular contraction pathways are counterbalanced by enhanced ETa-mediated vascular relaxation pathways, thus preventing excessive increases in vascular resistance and arterial pressure. Accordingly, chronic blockade of Eta receptors during high salt diet will not only decrease vascular relaxation, but also enhance vascular reactivity leading to increased vascular resistance and salt-sensitive hypertension. The decreased vascular relaxation occurs as a result of inhibition of the endothelium-dependent nitric oxide-cGMP, prostacyclincAMP and/or hyperpolarizing factor pathway. The increased vascular reactivity occurs as a result of increased [Ca2+]i and protein kinase C activity in vascular smooth muscle. The increases in vascular reactivity, [Ca2+]i and PKC activity during chronic blockade of ETa receptors and high salt diet occur as a result of unbalanced stimulation of ETA receptors. To test this hypothesis chronically-instrumented rats on normal and high sodium diets and nontreated or treated with ETa and ETA receptor antagonists will be used. Integrated analysis will be used to investigate the relation between ETa-mediated vascular relaxation and ETA-mediated vascular contraction in isolated renal vessels, and the arterial pressure in vivo. These studies should help understand better the vascular protective mechanisms during high salt diet in normal individuals and the pathophysiological basis of the increased vascular resistance in salt-sensitive hypertension.

描述（由申请人提供）：高盐饮食通常与盐敏感个体的血管阻力和动脉压增加有关。然而，在正常个体中，高盐饮食不会显著增加动脉压，这表明可能的血管保护机制。内皮素-1（ET-1）激活ETA和ETa受体。尽管ETA受体在血管收缩和高血压中的作用已被广泛研究，但ETA受体在调节血管功能和动脉压，特别是在高盐饮食期间的重要性尚不清楚。初步数据表明，ET-1的产生在高钠摄入量期间增强。数据还表明，ETa受体的慢性阻断导致盐敏感型高血压;然而，涉及的血管和细胞机制尚不清楚。本提案的总体目标是检验以下假设：正常情况下，在高盐饮食期间，ET-1产生的增加及其ETA介导的血管收缩途径的增强被增强的ETA介导的血管舒张途径抵消，从而防止血管阻力和动脉压过度增加。因此，在高盐饮食期间Eta受体的慢性阻断不仅会降低血管舒张，而且会增强血管反应性，导致血管阻力增加和盐敏感性高血压。血管舒张的减少是由于内皮依赖性一氧化氮-cGMP、前列环素AMP和/或超极化因子途径的抑制而发生的。血管反应性增加是由于血管平滑肌中[Ca 2 +]i和蛋白激酶C活性增加而发生的。在长期阻断ETA受体和高盐饮食期间，血管反应性、[Ca ~（2+）]i和PKC活性的增加是由于ETA受体的不平衡刺激的结果。为了检验这一假设，将使用正常和高钠饮食且未处理或用ETA和ETA受体拮抗剂处理的慢性仪器大鼠。 综合分析将用于研究离体肾血管中ETA介导的血管舒张和ETA介导的血管收缩与体内动脉压之间的关系。这些研究有助于更好地理解正常人高盐饮食时的血管保护机制以及盐敏感性高血压血管阻力增加的病理生理基础。