Fresh Human Osteochondral Allograft Preservation

新鲜人同种异体骨软骨移植物的保存

• 批准号: 7093013
• 负责人: WILLIAM D BUGBEE
• 金额: $ 7.54万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7093013
• 关键词: articular cartilage; ascorbate; biomechanics; cartilage disorder; cartilage metabolism; chondrocytes; clinical research; growth media; homologous transplantation; insulin; insulinlike growth factor; selenium; technology /technique development; temperature; tissue /organ preservation; transferrin

DESCRIPTION (provided by applicant): Damaged or diseased articular cartilage has limited intrinsic repair capabilities, and the natural history of affected joints is generally poor, leading to progressive arthrosis. The use of fresh human osteochondral allografts to replace articular cartilage defects and resurface areas of osteoarthrosis, and in the reconstruction of acquired osteochondral defects caused by trauma and disease, is an area of growing interest in the arthritis and orthopaedic community. A major obstacle to more wide-spread utilization of allografting is the inherent difficulties in procurement, processing, testing, and delivery of grafts; thus, the number of patients waiting for or eligible to undergo this procedure exceeds the amount of available tissue. This is primarily because the window of permissible storage time for this fresh tissue is narrow; and therefore, presently, these procedures can only be done in specialized centers. In the present study, we propose to assess chondrocyte viability, chondrocyte metabolic activity, cartilage matrix composition, and biomechanical properties within osteochondral plugs stored in different solutions. These solutions will be lactated Ringer's (to serve as a control), allograft storage media, allograft storage media plus fetal bovine serum, and allograft storage media containing defined media supplement insulin, transferrin and selenium (ITS); or vitamin C plus insulin-like growth factor-1. We also propose to investigate the effect of temperature on storage of osteochondral allografts, and the effect of the storage of osteochondral allografts with varying amounts of bone. We hypothesize that the more bone stored with articular cartilage, the greater the adverse effect on cartilage. This study should provide a framework in understanding the optimization of storage conditions for the preservation of osteochondral allografts, as well as a more complete understanding of the scientific basis for osteochondral allograft storage. The data should allow enhanced tissue banking and improve the availability of the limited supply of osteochondral allografts to fulfill the clinical need of these grafts in the treatment of individuals with articular cartilage injury and disease.

描述（由申请人提供）： 受损或患病的关节软骨的内在修复能力有限，受影响关节的自然病史通常很差，导致进行性关节炎。使用新鲜的人骨软骨同种异体移植物来代替骨关节炎的关节软骨缺陷和重新表面区域，在重建受创伤和疾病引起的骨软骨缺陷的重建方面是对关节炎和骨科社区的兴趣日益增长的领域。更广泛利用同种异体的主要障碍是采购，加工，测试和交付移植物的固有困难。因此，等待或有资格接受此过程的患者数量超过了可用组织的量。 这主要是因为此新鲜组织的允许存储时间窗口很狭窄。因此，目前，这些程序只能在专业中心进行。 在本研究中，我们建议评估软骨细胞的生存力，软骨细胞代谢活性，软骨基质组成和骨软骨塞中存储在不同溶液中的骨软骨塞内的生物力学特性。这些溶液将被乳酸（作为对照），同种异体移植培养基，同种异体移植培养基以及胎牛血清以及含有定义的培养基补充胰岛素，转移蛋白和硒的同种异体移植培养基（ITS）；或维生素C和胰岛素样生长因子1。我们还建议研究温度对骨软骨同种异体移植物的储存的影响，以及骨软骨同种异体移植物的储存影响不同。我们假设用关节软骨储存的骨骼越多，对软骨的不利影响就越大。这项研究应提供一个框架，以了解保存骨软骨同种异体移植的储存条件的优化，以及对骨软骨同种异体移植储存的科学基础的更完整理解。数据应允许增强组织库，并提高骨软骨同种异体移植物供应有限的可用性，以满足这些移植物的临床需求，以治疗关节软骨损伤和疾病的个体。

项目成果

Osteochondral allograft transplantation in cartilage repair: Graft storage paradigm, translational models, and clinical applications.

• DOI: 10.1002/jor.22998
• 发表时间: 2016-01
• 期刊: Journal of orthopaedic research : official publication of the Orthopaedic Research Society
• 作者: Bugbee WD;Pallante-Kichura AL;Görtz S;Amiel D;Sah R
• 通讯作者: Sah R