Mosquito-malaria interactions in the hemocoel

描述(申请人提供)：蚊子传播致病病原体。仅疟疾的病原体--疟原虫一项，每年就导致数百万人死亡。为了将疟原虫自然传播到脊椎动物宿主，这种寄生虫在蚊子媒介中经历了一系列强制性的发育和繁殖过程。在目前的应用中感兴趣的是蚊子血腔中疟原虫的造孢子期的生物学。初步实验已在冈比亚按蚊中鉴定出与埃及伊蚊唾液腺孢子虫受体同源的蛋白质家族。在这里，我们将刻画这些AN的时间和空间表达。冈比亚蛋白，并确定它们参与子孢子侵袭唾液腺的过程。此外，唾液腺侵袭是一个非常低效的过程。许多子孢子不能入侵并在血腔内迅速死亡。在这里，我们还将尝试描述蚊子杀灭子孢子的内源机制。了解寄生虫在媒介内完成其生命周期所使用的机制，以及媒介用于杀死寄生虫的机制，可能会导致新的传播控制策略。

DESCRIPTION (provided by applicant): Mosquitoes transmit disease-causing pathogens. Plasmodium parasites alone, the causative agents of malaria, kill millions of people each year. For natural transmission of Plasmodium to the vertebrate host, the parasite undergoes a series of obligatory developmental and propagative processes in a mosquito vector. Of interest in the current application is the biology of the sporogonic stages of Plasmodium in the mosquito hemocoel. Preliminary experiments have identified a family of proteins in the mosquito Anopheles gambiae with homology to an Aedes aegypti putative salivary gland sporozite receptor. Here, we will characterize the temporal and spatial expression of these An. gambiae proteins, and determine their involvement in salivary gland invasion by sporozoites. Furthermore, salivary gland invasion is a very inefficient process. Many sporozoites are unable to invade and rapidly die in the hemocoel. Here, we will also attempt to characterize the endogenous mechanisms used by mosquitoes to kill sporozoites. Understanding the mechanisms used by parasites to complete their life cycle inside the vector, and the mechanisms used by the vector to kill parasites, may lead to novel transmission control strategies.