Mosquito-malaria interactions in the hemocoel

描述（由申请人提供）：蚊子传播致病病原体。仅疟原虫一种疟疾病原体，每年就夺走数百万人的生命。为了将疟原虫自然传播到脊椎动物宿主，寄生虫在蚊子载体中经历一系列强制性发育和繁殖过程。在本申请中感兴趣的是蚊子血腔中疟原虫孢子生殖阶段的生物学。初步实验已经鉴定了冈比亚按蚊中的一个蛋白质家族，其与埃及伊蚊假定的唾液腺孢子受体具有同源性。在这里，我们将描述这些An的时间和空间表达。冈比亚蛋白，并确定它们参与子孢子的唾液腺入侵。此外，唾液腺侵入是一个非常低效的过程。许多子孢子不能侵入血腔并迅速死亡。在这里，我们也将试图描述蚊子杀死子孢子的内源性机制。了解寄生虫在载体内完成其生命周期所使用的机制，以及载体杀死寄生虫所使用的机制，可能会导致新的传播控制策略。

DESCRIPTION (provided by applicant): Mosquitoes transmit disease-causing pathogens. Plasmodium parasites alone, the causative agents of malaria, kill millions of people each year. For natural transmission of Plasmodium to the vertebrate host, the parasite undergoes a series of obligatory developmental and propagative processes in a mosquito vector. Of interest in the current application is the biology of the sporogonic stages of Plasmodium in the mosquito hemocoel. Preliminary experiments have identified a family of proteins in the mosquito Anopheles gambiae with homology to an Aedes aegypti putative salivary gland sporozite receptor. Here, we will characterize the temporal and spatial expression of these An. gambiae proteins, and determine their involvement in salivary gland invasion by sporozoites. Furthermore, salivary gland invasion is a very inefficient process. Many sporozoites are unable to invade and rapidly die in the hemocoel. Here, we will also attempt to characterize the endogenous mechanisms used by mosquitoes to kill sporozoites. Understanding the mechanisms used by parasites to complete their life cycle inside the vector, and the mechanisms used by the vector to kill parasites, may lead to novel transmission control strategies.