Color-coded transposor-mediated rat mutagenesis

描述（由申请人提供）： 大鼠是研究哺乳动物生物学的重要实验动物。这些啮齿类动物特别适用于生理学、神经行为学、器官移植和毒理学研究。此外，现在还可以获得大鼠的特殊遗传和基因组资源，包括一个基因组，该基因组已被测序，目前的组装和注释预测了23，400个基因（www.ensembl.org/Rattus_norvegjcus）和50，000多个表达序列标签（EST）。大鼠模型系统现在已准备好进行功能基因组研究。然而，一个特别有价值的资源，以促进基因功能的研究，这是非常缺乏的大鼠系统是一个巨大的收集突变体和新的突变的连续来源。虽然有可能常规地产生转基因大鼠来表达外源基因，但该物种没有胚胎干（ES）细胞可用于产生小鼠中常规的靶向突变。容易产生新的突变大鼠品系的能力将显着推进使用这种中心实验室动物进行生物医学研究。该提案的主要目的是通过转基因大鼠产生睡美人转座子诱变系统，作为研究界免费获得的资源，以容易地产生和分子鉴定大鼠中的新突变。

DESCRIPTION (provided by applicant): Rats are very important laboratory animals for studying mammalian biology. These rodents are especially useful for physiological, neurobehavioral, organ transplantation, and toxicological studies. In addition, exceptional genetic and genomic resources are now available for the rat, including a genome that has been sequenced with a current assembly and annotation predicting 23,400 genes (www.ensembl.org/Rattus_norvegjcus) and more than 50,000 expressed sequence tags (ESTs). The rat model system is now primed for functional genomic studies. However, one particularly valuable resource to facilitate studies of gene function that is sorely lacking in the rat system is a large collection of mutants and a continuous source of new mutations. Although it is possible to routinely generate transgenic rats to express exogenous genes, there are no embryonic stem (ES) cells for this species that could be used to generate targeted mutations that is routine in the mouse. The ability to easily generate new mutant rat strains would significantly advance the use of this central laboratory animal for biomedical research. The primary objective of this proposal is to generate a Sleeping Beauty transposon mutagenesis system through transgenic rats to serve as a freely available resource to the research community to easily generate and molecularly identify new mutations in rats.