INFRASTRUCTIRE DEVELOPMENT GRANT FOR CORNEA RESEARCH

用于角膜研究的基础设施发展补助金

• 批准号: 7057227
• 负责人: JERRY NIEDERKORN
• 金额: $ 22.87万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7057227
• 关键词: biomedical equipment resource; biomedical facility; cornea; rod cell; tissue /cell preparation; tissue /organ preservation

DESCRIPTION (provided by applicant): An Infrastructure Development Grant is proposed to support cornea and anterior segment research in the Department of Ophthalmology at the University of Texas Southwestern Medical Center. Cornea and anterior segment-related research has been a major focus of our department over the past 20 years. These research programs share a common reliance on two technologies: 1) tissue culture and monoclonal antibody production and 2) imaging. Establishing core facilities in these two areas would allow investigators in the Department of Ophthalmology to consolidate their research activities and thereby save resources, time, and manpower. Two modules are proposed: 1) Tissue Culture/Monoclonal Antibody Module; and 2) Microscopic Imaging Module. The Tissue Culture/Monoclonal Antibody Module will provide: a) routine maintenance of continuous and primary cell lines; b) maintain a cell and tumor bank of cryopreserved cells; c) produce monoclonal antibodies from existing hybridomas or hybridomas generated de novo; and d) provide regular screening of cell cultures for endotoxin and mycoplasma contamination. The Microscopic Imaging Module will provide: a) routine histology and immunohistochemistry; b) conventional light and fluorescent microscopy; c) laser scanning confocal and multiphoton confocal microscopy; d) in vivo confocal microscopy; e) quantitative morphometry; and f) publication and printing. The goal of the Infrastructure Development Core Grant is to provide central facilities for commonly utilized technologies for existing research programs and to provide resources for pilot studies and developmental studies that will hopefully culminate in new research initiatives in our department. These facilities will save time, energy, and resources while enhancing the success of seven NEI-sponsored research programs at U.T. Southwestern Medical Center.

描述（由申请人提供）：基础设施发展补助金拟用于支持德克萨斯大学西南医学中心眼科系的角膜和眼前节研究。在过去的20年里，角膜和前节相关的研究一直是我们部门的主要重点。这些研究计划共同依赖于两种技术：1）组织培养和单克隆抗体生产以及2）成像。在这两个领域建立核心设施将使眼科系的研究人员能够巩固他们的研究活动，从而节省资源，时间和人力。 提出了两个模块：1）组织培养/单克隆抗体模块;和2）显微成像模块。组织培养/单克隆抗体模块将提供：a）连续和原代细胞系的常规维护; B）保存冻存细胞的细胞和肿瘤库; c）从现有杂交瘤或从头生成的杂交瘤中产生单克隆抗体; d）提供细胞培养物的内毒素和支原体污染的定期筛查。显微成像模块将提供：a）常规组织学和免疫组织化学; B）常规光学和荧光显微镜检查; c）激光扫描共聚焦和多光子共聚焦显微镜检查; d）体内共聚焦显微镜检查; e）定量形态测定;以及f）出版和打印。 基础设施发展核心补助金的目标是为现有研究计划的常用技术提供中央设施，并为试点研究和发展研究提供资源，这些研究有望在我们部门的新研究计划中达到高潮。这些设施将节省时间，能源和资源，同时提高了七个NEI赞助的研究计划在德州大学的成功。西南医学中心