INFRASTRUCTIRE DEVELOPMENT GRANT FOR CORNEA RESEARCH

描述(由申请者提供)：提议设立基础设施发展基金，以支持德克萨斯大学西南医学中心眼科眼科的角膜和前段研究。在过去的20年里，角膜和前段相关的研究一直是我科的主要工作重点。这些研究项目共同依赖于两项技术：1)组织培养和单抗生产；2)成像。在这两个领域建立核心设施将使眼科的研究人员能够整合他们的研究活动，从而节省资源、时间和人力。 提出了两个模块：1)组织培养/单抗模块；2)显微成像模块。组织培养/单抗模块将提供：a)连续和原代细胞系的常规维护；b)维持冷冻细胞的细胞库和肿瘤库；c)从现有的杂交瘤或从头产生的杂交瘤产生单抗；以及d)定期对细胞培养物进行内毒素和支原体污染的筛查。显微成像模块将提供：a)常规组织学和免疫组织化学；b)常规光学和荧光显微镜；c)激光扫描共焦显微镜和多光子共焦显微镜；d)活体共焦显微镜；e)定量形态测量；以及f)出版和印刷。 基础设施发展核心补助金的目标是为现有研究计划中常用的技术提供中央设施，并为试点研究和发展研究提供资源，这些研究有望在我们系内产生新的研究倡议。这些设施将节省时间、能源和资源，同时提高德克萨斯大学西南医学中心由NEI赞助的七个研究项目的成功。

DESCRIPTION (provided by applicant): An Infrastructure Development Grant is proposed to support cornea and anterior segment research in the Department of Ophthalmology at the University of Texas Southwestern Medical Center. Cornea and anterior segment-related research has been a major focus of our department over the past 20 years. These research programs share a common reliance on two technologies: 1) tissue culture and monoclonal antibody production and 2) imaging. Establishing core facilities in these two areas would allow investigators in the Department of Ophthalmology to consolidate their research activities and thereby save resources, time, and manpower. Two modules are proposed: 1) Tissue Culture/Monoclonal Antibody Module; and 2) Microscopic Imaging Module. The Tissue Culture/Monoclonal Antibody Module will provide: a) routine maintenance of continuous and primary cell lines; b) maintain a cell and tumor bank of cryopreserved cells; c) produce monoclonal antibodies from existing hybridomas or hybridomas generated de novo; and d) provide regular screening of cell cultures for endotoxin and mycoplasma contamination. The Microscopic Imaging Module will provide: a) routine histology and immunohistochemistry; b) conventional light and fluorescent microscopy; c) laser scanning confocal and multiphoton confocal microscopy; d) in vivo confocal microscopy; e) quantitative morphometry; and f) publication and printing. The goal of the Infrastructure Development Core Grant is to provide central facilities for commonly utilized technologies for existing research programs and to provide resources for pilot studies and developmental studies that will hopefully culminate in new research initiatives in our department. These facilities will save time, energy, and resources while enhancing the success of seven NEI-sponsored research programs at U.T. Southwestern Medical Center.