Vision Research Infrastructure Development Grant

描述(由申请人提供)：克利夫兰临床基金会(CCF)科尔眼科研究所的视力研究计划提出了一个研究基础设施发展计划，该计划将促进对眼睛和视觉系统的结构、发育和功能的研究，在健康和疾病方面，目的是预防或治疗导致失明的眼睛疾病。受益于这一支持的研究人员使用了最先进的研究技术，包括基因组学、蛋白质组学、电生理学以及细胞和分子生物学。拟议的两个资源模块将有助于实现这一目标： 蛋白质组学/蛋白质化学资源模块(利用氨基酸分析、二维凝胶电泳、质谱学为定制研究需求提供蛋白质鉴定方面的帮助。) 显微镜/组织学资源模块(使用共聚焦显微镜、电子显微镜、明场、暗场、相差或荧光显微镜、免疫细胞化学和计算机辅助图像分析的组织分析的组织学支持。) 这是由首席调查员和其他15名受资助的视觉科学家提交的新申请。这些科学家包括六名拥有八项符合条件的NEI奖学金的科学家。其他九名基础和临床研究人员的资金来自其他来源。在后者中，有四个已经提交了NEI R01申请，另外两个计划在未来这样做。CCF承诺通过建造新的实验室空间、为资源模块购买各种新设备、为管理补助金的管理员提供薪金支助以及为调查员薪金提供部分支助来支持愿景研究核心中心。这项愿景研究基础设施发展补助金将为这些研究人员带来所需的重要核心资源服务。他们的研究活动将通过这些服务得到加强，他们的协作互动将通过该计划得到加强和扩展。

DESCRIPTION (provided by applicant): The vision research program of the Cleveland Clinic Foundation's (CCF) Cole Eye Institute proposes a program of research infrastructure development that will facilitate the study of the structure, development, and function of the eye and visual system in health and disease, with the goal of preventing or curing disorders of the eye that lead to blindness. The investigators benefiting from this support use the most sophisticated research techniques available, including those of genomics, proteomics, electrophysiology, and cell and molecular biology. The two resource modules that are proposed will help achieve this goal: I. Proteomics/Protein Chemistry Resource Module (Assistance in protein identification using amino acid analysis, 2D gel electrophoresis, mass spectroscopy for custom research needs.) II. Microscopy/Histology Resource Module (Histology support for tissue analysis using confocal microscopy, electron microscopy, brightfield, darkfield, phase contrast or fluorescence microscopy, immunocytochemistry, and computer-aided image analysis.) This is a new application submitted by the Principal Investigator and 15 other funded vision scientists. The scientists include six with eight eligible NEI grants. The nine other basic and clinical investigators are funded by other sources. Of the latter, four have submitted NEI R01 applications, and two others plan to do so in the future. The CCF has committed to support the Vision Research Core Center by constructing new laboratory space; purchasing a wide range of new equipment for the resource modules; providing salary support for an administrator to manage the grant; and providing partial support for investigator salaries. This Vision Research Infrastructure Development Grant will bring important Core Resource services needed by these investigators. Their research activities will be enhanced by these services and their collaborative interactions will be strengthened and extended through this program.