Vision Research Infrastructure Development Grant

愿景研究基础设施发展补助金

• 批准号: 7434313
• 负责人: JOE Gilbert HOLLYFIELD
• 金额: $ 22.95万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7434313
• 关键词: Administrator; Amino Acids; Blindness; Clinic; Clinical Investigator; Commit; Computer Assisted; Confocal Microscopy; Custom; Development; Disease; Electron Microscopy; Electrophysiology (science); Equipment; Eye; Eye diseases; Fluorescence Microscopy; Foundations; Funding; Future; Genomics; Goals; Grant; Health; Histology; Image Analysis; Institutes; Laboratories; Lead; Mass Spectrum Analysis; Microscopy; Molecular and Cellular Biology; Phase; Principal Investigator; Protein Chemistry; Proteins; Proteomics; Range; Research; Research Activity; Research Infrastructure; Research Personnel; Research Technics; Resources; Scientist; Services; Source; Structure; Tissues; Two-Dimensional Gel Electrophoresis; Vision; Vision research; Visual system structure; Wages; immunocytochemistry; prevent; programs

DESCRIPTION (provided by applicant): The vision research program of the Cleveland Clinic Foundation's (CCF) Cole Eye Institute proposes a program of research infrastructure development that will facilitate the study of the structure, development, and function of the eye and visual system in health and disease, with the goal of preventing or curing disorders of the eye that lead to blindness. The investigators benefiting from this support use the most sophisticated research techniques available, including those of genomics, proteomics, electrophysiology, and cell and molecular biology. The two resource modules that are proposed will help achieve this goal: I. Proteomics/Protein Chemistry Resource Module (Assistance in protein identification using amino acid analysis, 2D gel electrophoresis, mass spectroscopy for custom research needs.) II. Microscopy/Histology Resource Module (Histology support for tissue analysis using confocal microscopy, electron microscopy, brightfield, darkfield, phase contrast or fluorescence microscopy, immunocytochemistry, and computer-aided image analysis.) This is a new application submitted by the Principal Investigator and 15 other funded vision scientists. The scientists include six with eight eligible NEI grants. The nine other basic and clinical investigators are funded by other sources. Of the latter, four have submitted NEI R01 applications, and two others plan to do so in the future. The CCF has committed to support the Vision Research Core Center by constructing new laboratory space; purchasing a wide range of new equipment for the resource modules; providing salary support for an administrator to manage the grant; and providing partial support for investigator salaries. This Vision Research Infrastructure Development Grant will bring important Core Resource services needed by these investigators. Their research activities will be enhanced by these services and their collaborative interactions will be strengthened and extended through this program.

描述（由申请人提供）：克利夫兰诊所基金会（CCF）科尔眼科研究所的视力研究计划提出了一项研究基础设施发展计划，该计划将促进健康和疾病中眼睛和视觉系统的结构，发育和功能的研究，目的是预防或治疗导致失明的眼睛疾病。受益于这种支持的研究人员使用最先进的研究技术，包括基因组学，蛋白质组学，电生理学以及细胞和分子生物学。拟议的两个资源模块将有助于实现这一目标： I.蛋白质组学/蛋白质化学资源模块（使用氨基酸分析，2D凝胶电泳，质谱法进行蛋白质鉴定，以满足定制研究需求。） 二.显微镜/组织学资源模块（使用共聚焦显微镜、电子显微镜、明视野、暗视野、相差或荧光显微镜、免疫细胞化学和计算机辅助图像分析进行组织学支持。） 这是由首席研究员和其他15位受资助的视觉科学家提交的新申请。这些科学家包括六名获得八项符合条件的NEI赠款的科学家。其他9名基础和临床研究人员由其他来源资助。在后者中，有四家已经提交了NEI R 01申请，另外两家计划在未来这样做。CCF致力于通过建设新的实验室空间来支持视觉研究核心中心;为资源模块购买各种新设备;为管理员管理赠款提供工资支持;并为研究人员提供部分工资支持。这项愿景研究基础设施发展赠款将带来这些研究人员所需的重要核心资源服务。他们的研究活动将通过这些服务得到加强，他们的合作互动将通过该计划得到加强和扩展。

项目成果

Betacellulin induces increased retinal vascular permeability in mice.

• DOI: 10.1371/journal.pone.0013444
• 发表时间: 2010-10-18
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Anand-Apte B;Ebrahem Q;Cutler A;Farage E;Sugimoto M;Hollyfield J;Folkman J
• 通讯作者: Folkman J

Mutation screen of beta-crystallin genes in 274 patients with age-related macular degeneration.

274 例年龄相关性黄斑变性患者的 β-晶状体蛋白基因突变筛查。

• DOI: 10.3109/13816810.2010.486774
• 发表时间: 2010
• 期刊: Ophthalmic genetics
• 影响因子: 1.2
• 作者: Sturgill,GwenM;Bala,Elisa;Yaniglos,StaciaS;Peachey,NealS;Hagstrom,StephanieA
• 通讯作者: Hagstrom,StephanieA

Protein partners of dynamin-1 in the retina.

• DOI: 10.1017/s0952523813000138
• 发表时间: 2013-07
• 期刊: VISUAL NEUROSCIENCE
• 影响因子: 1.9
• 作者: Grossman, Gregory H.;Ebke, Lindsey A.;Beight, Craig D.;Jang, Geeng-Fu;Crabb, John W.;Hagstrom, Stephanie A.
• 通讯作者: Hagstrom, Stephanie A.

Immunocytochemical evidence of Tulp1-dependent outer segment protein transport pathways in photoreceptor cells.

光感受器细胞中依赖TULP1的外部段蛋白转运途径的免疫细胞化学证据。

• DOI: 10.1016/j.exer.2011.08.005
• 发表时间: 2011-11
• 期刊: EXPERIMENTAL EYE RESEARCH
• 影响因子: 3.4
• 作者: Grossman, Gregory H.;Watson, Rao F.;Pauer, Gayle J. T.;Bollinger, Kathryn;Hagstrom, Stephanie A.
• 通讯作者: Hagstrom, Stephanie A.

Isolating photoreceptor compartment-specific protein complexes for subsequent proteomic analysis.

分离光感受器区室特异性蛋白质复合物以进行后续蛋白质组分析。

• DOI: 10.1007/978-1-4614-0631-0_89
• 发表时间: 2012
• 期刊: Advances in experimental medicine and biology
• 作者: Grossman,GregoryH;Pauer,GayleJT;Hoppe,George;Hagstrom,StephanieA
• 通讯作者: Hagstrom,StephanieA