PROTEIN AND HISTOLOGY MINICORE GRANT FOR VISION RESEARCH

描述（由申请人提供）：密苏里州大学眼科系的视觉研究学院建议建立两个研究模块，以支持该部门正在进行的活动。蛋白质表达和分析模块（模块#1）将在Dr. Sharma的指导下设置，用于晶体蛋白表达、分离和分析。该模块将支持需要大量晶体蛋白的研究者的需求，需要帮助分析透镜或视网膜成分，以及需要培养整个晶状体或透镜上皮细胞。透镜上皮细胞的建立将使我们能够在体内条件下观察信号转导和基因表达。蛋白质分析模块的建立将使我们能够共享现有资源，并提高我们为最近加入的教师提供支持的能力，以及我们计划在使命增强计划下招募的新员工。组织学和图像分析模块（模块#2）将用于分析转基因动物或病理标本或供体组织的眼组织样本。该模块将由Reneker博士指导。它将促进部门内NEI支持项目之间的持续合作。此外，该模块将为我们的新教师发起的研究项目提供急需的支持。因此，本提案的主要目标是： a）加强密苏里州大学医学院视觉科学研究的研究基础设施， B）增加联合研究项目的机会，并为联合项目提供后勤支持， （三）支持新视野研究院的需求。

DESCRIPTION (provided by applicant): The vision research faculty in the Department of Ophthalmology at the University of Missouri propose to establish two research modules to support the ongoing activities in the department. The Protein expression and analysis module (Module #1) will be setup under the direction of Dr. Sharma for crystallin expression, isolation, and analysis. This module will support the need of investigators who will need large quantities of crystallins, need assistance in the analysis of lens or retinal constituents and the need to culture of whole lenses or lens epithelial cells. The establishment of lens epithelial cells in culture will allow us to look into signal transduction and gene expression under in vivo conditions. The establishment of protein analysis module will allow us to share the existing resources and increase our ability to offer support to recently joined faculty and the new faulty we plan on recruiting under the mission enhancement program. The Histology and image analysis module (Module #2) will be a facility for analyzing eye tissue samples from transgenic animals or pathologic specimens or donor tissues. This module will be directed by Dr. Reneker. It will foster the ongoing collaboration between the NEI supported projects within the department. In addition, the module will offer the much needed support for the research projects initiated by our new faculty. Therefore the main objectives of this proposal are to: a) enhance research infrastructure for vision science research at University of Missouri School of Medicine, b) enhance the opportunities for joint research projects and provide logistical support for joint projects, c) support the needs of new vision research faculty.