Exposure to Mixtures of Emerging Contaminants in the Environment - Are Communities in Uganda at Health Risk?- A Case Study of Mbarara City.

项目摘要/摘要：鲁比齐河是姆巴拉拉市和 环境受到人为污染的威胁。初步研究发现了杀虫剂， 多环芳烃和重金属的浓度高于世卫组织的饮用水 水建议，使超过91,964名城市居民暴露在污染物中。污染物暴露 与非传染性疾病(NCDs)和新兴污染物(EP)有关构成新的 对人类健康的威胁。还没有研究完全表征鲁瓦济河或废水中的EPs 污水处理厂(WWTP)在其集水区，然而EPP和废水之间有着千丝万缕的联系。它 假设姆巴拉市不断变化的人类活动增加了 鲁比齐河中EPs的浓度，使居民通过以下途径接触到更高的非传染性疾病风险 代谢组的扰动。这项研究将为干预策略提供信息，以减少 LMICs中的环境引起的疾病有助于实现NIEHS目标和乌干达的健康 预防、缓解和控制疾病的优先事项。应聘者：我有研究方面的技能 方法论、数据分析、科学陈述和手稿写作 学士、硕士和博士的培训和研究工作，到目前为止发表了23篇论文。这款K43 格兰特将确保我在以下方面获得培训和指导：先进的研究方法、管理 大数据、研究管理、负责任的研究行为和拨款撰写。这 拟议的职业发展计划将建立在我之前的培训基础上，以帮助我 通过五个培训目标成为独立调查员：1)执行高级 定性和定量1H-核磁共振和LC-MS代谢组学；2)高级统计 分析；3)在生物过程的背景下解释代谢组学数据；4) 加强领导和专业技能；5)组织病理学在慢性疾病中的应用 暴露研究。导师和环境：这项计划将受益于物质和人类 姆巴拉拉科技大学提供的资源[通过Dr. Tusubia和Alele教授(生物化学家和毒物学家)]，圣地亚哥大学和 作者声明：Makerere；访问数/每百万人：Reach for[by].研究：我将(I)识别和量化 利用色谱技术和废水对鲁伊齐河及其流域中的污水处理厂进行研究 流行病学方法。(2)将通过代谢组学研究暴露于EPs的影响和 组织病理学即将来临。我的短期目标是确定相关的候选代谢物 虽然我的长期目标是在环境领域发展事业，但我接触到了环保产品 毒物学。在这项支持结束时，我将申请一辆R01。

Project Summary/Abstract: River Rwizi the main source of water for Mbarara City and surroundings is threatened by anthropogenic pollution. Preliminary studies detected pesticides, polycyclic aromatic hydrocarbons and heavy metals at concentrations higher than WHO drinking water recommendations, exposing over 91,964 City residents to pollutants. Pollutant exposure is linked to Non-Communicable Diseases (NCDS) and emerging pollutants (EPs) pose new threats to human health. No study has fully characterized EPs in River Rwizi or wastewater treatment plants (WWTPs) in its catchment yet EPs and wastewater have an inextricable link. It is hypothesized that the changing anthropogenic activities in Mbarara City have increased the concentrations of EPs in River Rwizi, exposing residents to higher risks of NCDs through perturbations of the metabolome. This study will inform intervention strategies to reduce environmentally induced diseases in LMICs contributing to NIEHS goals and Uganda’s health priorities of disease prevention, mitigation and control. Candidate: I have skills in research methodology, data analysis, scientific presentation, and manuscript writing accrued during my BS, MSc and PhD training and research works that resulted in 23 publications so far. This K43 grant will secure me training and mentorship in: advanced research methodology, management of large data, research administration, responsible conduct of research, and grant writing. This proposed career development plan will build on my previous training to facilitate me to become an independent investigator through five training goals: 1) execution of advanced qualitative and quantitative 1H-NMR and LC-MS metabolomics; 2) advanced statistical analyses; 3) interpretation of metabolomics data in the context of biological processes; 4) strengthening leadership and professional skills; and 5) application of histopathology to chronic exposure studies. Mentors and Environment: This plan will benefit from physical and human resources available through Mbarara University of Science and Technology [through Dr. Tusubira and Prof. Alele (Biochemist and Toxicologist)], the Universities of San Diego and Makerere [Profs. Bolender and Ntale, Chemists]. Research: I will (i) Identify and quantify EPs in River Rwizi and in WWTPs in its catchment using chromatographic techniques and Wastewater Epidemiology approach. (ii) The effect of exposure to EPs will be studied by metabolomics and histopathology approaches. My short-term goal is to identify candidate metabolites associated with exposure to EPs while my long term goal is to develop a career in Environmental toxicology. Towards the end of this support I will apply for an R01.