Exposure to Mixtures of Emerging Contaminants in the Environment - Are Communities in Uganda at Health Risk?- A Case Study of Mbarara City.

接触环境中新兴污染物的混合物 - 乌干达的社区面临健康风险吗？ - 姆巴拉拉市的案例研究。

• 批准号: 10732272
• 负责人: Grace Birungi
• 金额: $ 10.81万
• 依托单位: MBARARA UNIVERSITY/SCIENCE/ TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-27 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10732272
• 关键词: Adverse effects; Affect; Africa; African; Americas; Animals; Aromatic Polycyclic Hydrocarbons; Arsenic; Asia; Biological; Biological Markers; Biological Process; Cardiovascular Diseases; Case Study; Cessation of life; Chemicals; Chromatography; Chronic; Cities; Communities; Control Groups; Country; Data; Data Analyses; Developed Countries; Development; Development Plans; Diagnosis; Disease; Doctor of Philosophy; Ecosystem; Environment; Environmental Health; Europe; Exposure to; Fluorides; Goals; Grant; Health; Health Priorities; Heavy Metals; Histologic; Histopathology; Human; Human Resources; Industrialization; Intervention; Knowledge; Leadership; Link; Malignant Neoplasms; Mammals; Manuscripts; Mentors; Mentorship; Metabolic; Metabolism; Methods; Monitor; Morbidity - disease rate; Municipalities; Mus; National Institute of Environmental Health Sciences; Nitrates; Organ; Pathway interactions; Persons; Pesticides; Plants; Pollution; Prognosis; Public Health; Publications; Recommendation; Reporting; Research; Research Methodology; Research Personnel; Risk; Risk Assessment; Risk Estimate; Science; Secure; Serum; Source; Statistical Data Interpretation; Structure; Surface; System; Techniques; Technology; Testing; Training; Uganda; Universities; Water; Water Stress; Work; Writing; albino mouse; anthropogenesis; biomarker validation; candidate identification; career; career development; disorder prevention; disorder risk; drinking water; environmental toxicology; ground water; high risk; insight; low and middle-income countries; metabolic profile; metabolome; metabolomics; mortality risk; pollutant; prevent; responsible research conduct; skills; wastewater epidemiology

Project Summary/Abstract: River Rwizi the main source of water for Mbarara City and surroundings is threatened by anthropogenic pollution. Preliminary studies detected pesticides, polycyclic aromatic hydrocarbons and heavy metals at concentrations higher than WHO drinking water recommendations, exposing over 91,964 City residents to pollutants. Pollutant exposure is linked to Non-Communicable Diseases (NCDS) and emerging pollutants (EPs) pose new threats to human health. No study has fully characterized EPs in River Rwizi or wastewater treatment plants (WWTPs) in its catchment yet EPs and wastewater have an inextricable link. It is hypothesized that the changing anthropogenic activities in Mbarara City have increased the concentrations of EPs in River Rwizi, exposing residents to higher risks of NCDs through perturbations of the metabolome. This study will inform intervention strategies to reduce environmentally induced diseases in LMICs contributing to NIEHS goals and Uganda’s health priorities of disease prevention, mitigation and control. Candidate: I have skills in research methodology, data analysis, scientific presentation, and manuscript writing accrued during my BS, MSc and PhD training and research works that resulted in 23 publications so far. This K43 grant will secure me training and mentorship in: advanced research methodology, management of large data, research administration, responsible conduct of research, and grant writing. This proposed career development plan will build on my previous training to facilitate me to become an independent investigator through five training goals: 1) execution of advanced qualitative and quantitative 1H-NMR and LC-MS metabolomics; 2) advanced statistical analyses; 3) interpretation of metabolomics data in the context of biological processes; 4) strengthening leadership and professional skills; and 5) application of histopathology to chronic exposure studies. Mentors and Environment: This plan will benefit from physical and human resources available through Mbarara University of Science and Technology [through Dr. Tusubira and Prof. Alele (Biochemist and Toxicologist)], the Universities of San Diego and Makerere [Profs. Bolender and Ntale, Chemists]. Research: I will (i) Identify and quantify EPs in River Rwizi and in WWTPs in its catchment using chromatographic techniques and Wastewater Epidemiology approach. (ii) The effect of exposure to EPs will be studied by metabolomics and histopathology approaches. My short-term goal is to identify candidate metabolites associated with exposure to EPs while my long term goal is to develop a career in Environmental toxicology. Towards the end of this support I will apply for an R01.

项目概要/摘要：Rwizi河是姆巴拉拉市的主要水源， 周围环境受到人为污染的威胁。初步研究检测到杀虫剂， 多环芳烃和重金属浓度高于世卫组织饮用水浓度 超过91，964名城市居民暴露在污染物中。污染物暴露 与非传染性疾病（NCDS）和新出现的污染物（EP）有关， 威胁人类健康。没有任何研究充分描述鲁维济河或废水中的EP 在其集水区，环境产品和废水有着千丝万缕的联系。它 假设Mbarara市不断变化的人类活动增加了 在Rwizi河集中的环境生产物，使居民面临更高的非传染性疾病风险， 代谢组的扰动。这项研究将为干预战略提供信息，以减少 环境引起的疾病在中低收入国家有助于实现NIEHS目标和乌干达的健康 疾病预防、缓解和控制的优先事项。应聘者：我有研究方面的技能 方法论、数据分析、科学演示和手稿写作在我的过程中积累起来 学士，硕士和博士的培训和研究工作，导致23出版物至今。K43 赠款将确保我的培训和指导：先进的研究方法，管理 大数据，研究管理，负责任的研究行为，以及拨款写作。这 建议的职业发展计划将建立在我以前的培训基础上，以帮助我 通过五个培训目标成为独立调查员：1）执行高级 定性和定量1H-NMR和LC-MS代谢组学; 2）高级统计 分析; 3）在生物过程的背景下解释代谢组学数据; 4） 加强领导和专业技能; 5）组织病理学在慢性病中的应用 暴露研究。导师和环境：这个计划将受益于物理和人类 资源可通过姆巴拉拉科技大学[通过博士。 Tusubira和Alele教授（生物化学家和毒理学家）]，圣地亚哥大学和 Makerere [Profs. Bolender和Ntale，化学家]。研究：我将（一）确定和量化EP， Rwizi河及其集水区的污水处理厂中使用色谱技术和废水 流行病学方法。(ii)暴露于EP的影响将通过代谢组学研究， 组织病理学方法。我的短期目标是确定候选代谢物相关 我的长期目标是在环境保护领域发展职业生涯， 毒理学在此支持结束时，我将申请R 01。