Instrumentation Grant/Leica TCS SP2 Confocal Microscope

仪器 Grant/Leica TCS SP2 共焦显微镜

• 批准号: 6877291
• 负责人: Pedro Salas
• 金额: $ 50万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877291
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cell biology; confocal scanning microscopy; lasers

DESCRIPTION (provided by applicant): This is a revised application requesting funds to purchase a Leica TCS SP2 AOBS confocal microscopy system to support the research projects of a group of NIH-funded Investigators at the University of Miami School of Medicine, drawn from the Departments of Cell Biology & Anatomy, Molecular & Cellular Pharmacology, and Ophthalmology. Conventional laser scanning confocal microscopy (LSCM) is now a state-of-the-art technology for vital imaging and/or the acquisition of high-resolution optical sections from thick biological specimens. Recent advancements in this field allow real-time and multi-dimensional imaging, as well as high quality in vivo imaging. Thus this instrumentation is absolutely critical for current cutting edge research. The instrumentation requested will be dedicated primarily to a small but broad-based user group composed of molecular cell biologists and vision scientists drawn form three departments. Nine of these investigators (Key Personnel) have had extensive experience with the technology in their NIH-funded research. A number of additional investigators are new users for whom access will provide a broader range of approaches. It is estimated that the needs of this group will account for approximately 97% of instrumentation use. The remainder of the time will be made available to new investigators who join the group, and to back-up the institutional core facility, which is currently heavily overbooked. The new Leica instrument will be housed in the McKnight Analytic Imaging Core Facility. The facility will be directed and co-directed by two full-time faculty members and supervised by a dedicated technical manager who will have primary responsibility for maintaining the instrument and training new users. An advisory committee has been organized to oversee the use and maintenance of the instrument. Policies for the use and equitable sharing of the instrument, and a plan for its long-term maintenance have been established by the advisory committee. Strong institutional commitment provides ongoing support for the core facility and the instrumentation. About half of the members of the primary user group have extensive time needs for use of the instrumentation which cannot be satisfied by the single existing core facility on campus. The instrument chosen further satisfies specific technical needs that are not met by the existing instrumentation on campus. Improved access to this technology will greatly enhance the NIH-funded projects of the user group.

描述（由申请人提供）：这是一份修订后的申请，要求提供资金购买 Leica TCS SP2 AOBS 共焦显微镜系统，以支持迈阿密大学医学院由 NIH 资助的一组研究人员的研究项目，这些研究人员来自细胞生物学与解剖学、分子与细胞药理学以及眼科学系。传统的激光扫描共焦显微镜 (LSCM) 现在是一种用于生命成像和/或从厚生物样本中获取高分辨率光学切片的最先进技术。该领域的最新进展允许实时和多维成像以及高质量的体内成像。因此，该仪器对于当前的前沿研究绝对至关重要。所需的仪器将主要致力于一个小而广泛的用户组，该用户组由来自三个部门的分子细胞生物学家和视觉科学家组成。其中九名研究人员（关键人员）在 NIH 资助的研究中拥有丰富的技术经验。许多额外的调查员是新用户，他们的访问将为他们提供更广泛的方法。据估计，这一群体的需求将占仪器使用量的约97%。剩余的时间将提供给加入该小组的新调查员，并支持目前严重超额预订的机构核心设施。新的徕卡仪器将安装在麦克奈特分析成像核心设施中。该设施将由两名全职教职人员指导和共同指导，并由一名专门的技术经理监督，该经理将主要负责维护仪器和培训新用户。已经成立了一个咨询委员会来监督该仪器的使用和维护。咨询委员会制定了该仪器的使用和公平分享政策以及长期维护计划。强有力的机构承诺为核心设施和仪器提供持续的支持。主要用户组中大约一半的成员需要大量的时间来使用仪器，而校园内现有的单一核心设施无法满足这一需求。所选仪器进一步满足了校园现有仪器无法满足的特定技术需求。改进该技术的获取将极大地增强 NIH 资助的用户群体项目。

项目成果

Proteomics of the nucleus ovoidalis and field L brain regions of zebra finch.

斑胸草雀卵圆核和 L 脑区的蛋白质组学。

• DOI: 10.1021/pr7008687
• 发表时间: 2008
• 期刊: Journal of proteome research
• 影响因子: 4.4
• 作者: Benjamin,Alexander;Kashem,Moses;Cohen,Camille;CaldwellBusby,JenniferA;Salgado-Commissariat,Delanthi;Helekar,SantoshA;Bhattacharya,SanjoyK
• 通讯作者: Bhattacharya,SanjoyK