Acquisition of a Transmission Electron Microscope to Reactivate Facility

购置透射电子显微镜以重新启动设施

• 批准号: 8247527
• 负责人: Pedro Salas
• 金额: $ 39.6万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247527
• 关键词: Accounting; Advisory Committees; Confocal Microscopy; Contract Services; Core Facility; Electron Microscope; Electron Microscopy; Faculty; Funding; Future; Housing; Improve Access; Institution; Maintenance; Medicine; Policies; Request for Applications; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Schools; Technology; Time; Training; United States National Institutes of Health; Universities; Wages; digital imaging; experience; instrument; instrumentation; member; nanometer; transmission process

DESCRIPTION (provided by applicant): This is an application requesting funds to purchase a Jeol JEM-1400 Transmission Electron Microscope (TEM), to support the research projects of a group of NIH-funded investigators at the University of Miami Miller School Of Medicine. Some of these investigators have recently moved to this institution and have heavy needs for TEM that cannot be fulfilled by the available instruments. TEM is absolutely essential when high-resolution is needed, that cannot be achieved with standard confocal microscopy. All the investigators in the group need morphological readouts that require resolution within a few nanometers that can only be achieved by TEM. All the investigators in the group have had extensive experience with electron microscopy in their NIH-funded research. It is estimated that the needs of the core group of investigators in this application will account for 92% of the instrument time. A number of new additional investigators is expected to join the core group and a plan is put forth to train them and provide them with the expert support f a well-trained EM technician already in campus. The facility will be housed in fully renovated space already available and will be directed and co-directed by two full-time faculty members (the PI and another major user) and supervised by the technical manager mentioned above, who will have primary responsibility for maintaining the instrument and training new users. An advisory committee has been organized to oversee the use and maintenance of the instrument. Policies for the use and equitable sharing of the instrument, and a plan for its long-term maintenance have been established by the advisory committee. Strong institutional commitment provides ongoing support for the core facility and the instrumentation, and includes space renovation, 100% support for future service contracts and a fraction of the salary of the manager. The TEM needs of the primary user group cannot be satisfied by existing TEM instruments that are already heavily used and very outdated. None of the instruments in campus provide digital imaging. Improved access to this technology will greatly enhance the NIH-funded projects of the user group and possible future users in campus. In summary, this application seeks funding to replace a defunct shared resource that was in use for over 22 years.

描述（由申请人提供）：这是一份申请，要求资金购买Jeol JEM-1400透射电子显微镜（TEM），以支持迈阿密大学米勒医学院的一组NIH资助的研究人员的研究项目。其中一些研究人员最近搬到这个机构，并有大量的TEM的需要，不能满足现有的仪器。 当需要高分辨率时，TEM是绝对必要的，这是标准共焦显微镜无法实现的。小组中的所有研究人员都需要形态学读数，这些读数需要在 只有TEM才能达到几纳米。 小组里所有的调查员 在NIH资助的研究中有丰富的电子显微镜经验。据估计，核心组研究者在此应用中的需求将占仪器时间的92%。一些新的额外的调查人员预计将加入核心小组，并提出了一项计划，以培训他们，并为他们提供专家支持的训练有素的EM技术人员已经在校园。该设施将被安置在已经完全翻新的空间内，并将由两名全职教师（PI和另一名主要用户）指导和共同指导，并由上述技术经理监督，他将主要负责维护仪器和培训新用户。已组织了一个咨询委员会来监督该仪器的使用和维护。咨询委员会制定了使用和公平分享该工具的政策以及长期维护该工具的计划。强有力的机构承诺为核心设施和仪器仪表提供持续支持，包括空间改造，对未来服务合同的100%支持和经理工资的一小部分。TEM的主要用户群的需要不能满足现有的TEM仪器已经被大量使用，非常过时。校园里的仪器都不提供数字成像。改进对该技术的访问将大大增强用户组和校园中可能的未来用户的NIH资助项目。简而言之，这项申请寻求资金，以取代一个已使用超过22年的已不复存在的共享资源。