NCRR Shared Instrumentation/1H (13C/15N) 5mm Cold Probe

NCRR 共享仪器/1H (13C/15N) 5mm 冷探头

• 批准号: 6877294
• 负责人: Frederick W. Dahlquist
• 金额: $ 28.55万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2006-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877294
• 关键词: biomedical equipment purchase; cryoscience; nuclear magnetic resonance spectroscopy; protein structure function

DESCRIPTION (provided by applicant): Funding is requested for a cryogenically cooled probe, preamplifier and helium recovery system for our shared 600 MHz NMR spectrometer. The addition of this cooled probe will greatly facilitate the research of the several research groups that are using or plan to use modern NMR techniques in their work. These groups are working on a wide range of projects that include investigations of the structure, function and dynamics of the proteins involved in bacterial signaling during bacterial chemotaxis and related signaling pathways; protein folding dynamics and the nature of the unfolded state; the structure of self assembly of RNA-RNA recognition units, the coupling of protein dynamics and catalysis in the bacterial DNA cytosine methyltransferase, structural studies of the proteins involved in the epigenetic control of the expression of genes that encode proteins present in pyelonephritis-associated pili; the structure an assembly of tau protein; structural elucidation of marine siderophores; proteins and models of biomineralization; and kinetic analysis of the acetylcholine transporter.

描述(由申请者提供)：为我们共享的600 MHz核磁共振光谱仪申请资金，用于低温冷却探头、前置放大器和氦回收系统。这种冷却探头的加入将极大地促进正在或计划在工作中使用现代核磁共振技术的几个研究小组的研究。这些小组开展的广泛项目包括：调查细菌趋化过程中参与细菌信号传递的蛋白质的结构、功能和动态以及相关的信号通路；蛋白质折叠动力学和未折叠状态的性质；RNA-RNA识别单元的自组装结构；细菌DNA胞嘧啶甲基转移酶中蛋白质动力学和催化作用的耦合；细菌DNA胞嘧啶甲基转移酶中编码蛋白表达的表观遗传调控基因的结构研究；tau蛋白的组装；海洋铁载体的结构阐明；生物矿化的蛋白质和模型；以及乙酰胆碱转运体的动力学分析。

项目成果

Structural insights into the interaction between the bacterial flagellar motor proteins FliF and FliG.

细菌鞭毛运动蛋白 FliF 和 FliG 之间相互作用的结构见解。

• DOI: 10.1021/bi3004582
• 发表时间: 2012
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Levenson,Robert;Zhou,Hongjun;Dahlquist,FrederickW
• 通讯作者: Dahlquist,FrederickW

Solution structure of Escherichia coli PapI, a key regulator of the pap pili phase variation.

大肠杆菌 PapI 的溶液结构，它是乳头菌毛相位变化的关键调节因子。

• DOI: 10.1016/j.jmb.2006.10.066
• 发表时间: 2007
• 期刊: Journal of molecular biology
• 影响因子: 5.6
• 作者: Kawamura,Tetsuya;Le,LisaUyenK;Zhou,Hongjun;Dahlquist,FrederickW
• 通讯作者: Dahlquist,FrederickW