NCRR Shared Instrumentation/1H (13C/15N) 5mm Cold Probe

描述（由申请人提供）：资金要求为我们共享的600 MHz NMR光谱仪的低温冷却探头，氦气和氦气回收系统。这种冷却探针的添加将极大地促进正在使用或计划在工作中使用现代核磁共振技术的几个研究小组的研究。这些研究小组正在开展一系列广泛的项目，包括研究细菌趋化性和相关信号通路期间参与细菌信号传导的蛋白质的结构、功能和动力学;蛋白质折叠动力学和未折叠状态的性质; RNA-RNA识别单元的自组装结构，细菌DNA胞嘧啶甲基转移酶中蛋白质动力学和催化的耦合，参与编码肾盂肾炎相关皮利中存在的蛋白质的基因表达的表观遗传控制的蛋白质的结构研究; tau蛋白的结构和组装;海洋铁载体的结构阐明;蛋白质和生物矿化模型;乙酰胆碱转运蛋白的动力学分析。

DESCRIPTION (provided by applicant): Funding is requested for a cryogenically cooled probe, preamplifier and helium recovery system for our shared 600 MHz NMR spectrometer. The addition of this cooled probe will greatly facilitate the research of the several research groups that are using or plan to use modern NMR techniques in their work. These groups are working on a wide range of projects that include investigations of the structure, function and dynamics of the proteins involved in bacterial signaling during bacterial chemotaxis and related signaling pathways; protein folding dynamics and the nature of the unfolded state; the structure of self assembly of RNA-RNA recognition units, the coupling of protein dynamics and catalysis in the bacterial DNA cytosine methyltransferase, structural studies of the proteins involved in the epigenetic control of the expression of genes that encode proteins present in pyelonephritis-associated pili; the structure an assembly of tau protein; structural elucidation of marine siderophores; proteins and models of biomineralization; and kinetic analysis of the acetylcholine transporter.