NCRR Shared Instrumentation/1H (13C/15N) 5mm Cold Probe

描述（由申请人提供）：申请资金用于我们共享的600 MHz核磁共振光谱仪的低温冷却探针，前置放大器和氦回收系统。这种冷却探针的加入将极大地促进几个正在使用或计划在其工作中使用现代核磁共振技术的研究小组的研究。这些小组正在开展广泛的项目，包括研究细菌趋化过程中参与细菌信号传导的蛋白质的结构、功能和动力学以及相关的信号传导途径；蛋白质折叠动力学和未折叠态的性质；RNA-RNA识别单元的自组装结构，细菌DNA胞嘧啶甲基转移酶中蛋白质动力学的耦合和催化作用，肾盂肾炎相关毛中编码蛋白质的基因表达的表观遗传控制所涉及的蛋白质结构研究；其结构为tau蛋白的组装；海洋铁载体的结构解析生物矿化的蛋白质和模型；以及乙酰胆碱转运体的动力学分析。

DESCRIPTION (provided by applicant): Funding is requested for a cryogenically cooled probe, preamplifier and helium recovery system for our shared 600 MHz NMR spectrometer. The addition of this cooled probe will greatly facilitate the research of the several research groups that are using or plan to use modern NMR techniques in their work. These groups are working on a wide range of projects that include investigations of the structure, function and dynamics of the proteins involved in bacterial signaling during bacterial chemotaxis and related signaling pathways; protein folding dynamics and the nature of the unfolded state; the structure of self assembly of RNA-RNA recognition units, the coupling of protein dynamics and catalysis in the bacterial DNA cytosine methyltransferase, structural studies of the proteins involved in the epigenetic control of the expression of genes that encode proteins present in pyelonephritis-associated pili; the structure an assembly of tau protein; structural elucidation of marine siderophores; proteins and models of biomineralization; and kinetic analysis of the acetylcholine transporter.