ROS and NO interaction in the PVN during hypertension?

描述（由申请人提供）：本研究的目的是采用综合方法研究肾包膜高血压中介导并可能影响神经递质活性的信号变化，特别是NO和ROS之间的相互关系。为了实现这一目标，将提出三个具体假设：假设#1：存在NAD(P)H氧化酶，并且该酶的活性被PVN中的Ang II和谷氨酸增加，从而导致肾包层高血压的“超氧化物（O2-）增加”；假设2：肾包裹性高血压患者存在超氧化物歧化酶（SOD），其活性降低导致PVN中O2-升高和过氧化氢（H2O2）减少；假设3：在肾包层性高血压中，O2-升高，与NO结合，降低NO，最终降低抑制性神经递质GABA；PVN中GABA的减少导致动脉压升高。确定这些信号通路的变化以及它们如何相互作用以引起血压变化对于理解这些通路对神经递质活性和高血压的影响具有重要意义。

DESCRIPTION (provided by applicant): The goal of the proposed research is to use an integrative approach to examine the signaling changes, specifically the interrelationships between NO and ROS, that mediate and may influence neurotransmitter activity in renal wrap hypertension. To achieve this goal, three specific hypotheses will be addressed: Hypothesis #1: NAD(P)H oxidase is present and the activity of the enzyme is increased by Ang II and glutamate in the PVN, thus resulting in a" increase of superoxide (O2-) in renal wrap hypertension; Hypothesis #2: Superoxide dismutase (SOD) is present and its activity is decreased contributing to the increase in O2- and a reduction in hydrogen peroxide (H2O2) in the PVN in renal wrap hypertension; and Hypothesis #3: In renal wrap hypertension, there is elevated O2- which combines with NO, reducing NO and ultimately decreasing the inhibitory neurotransmitter GABA; the reduced GABA in the PVN contributes to the rise in arterial pressure. Identifying the changes in these signaling pathways and how they interact to elicit changes in blood pressure would be significantly important for understanding the impact that these pathways have on neurotransmitter activity and hypertension.