Nuclear envelope membrane proteins and nuclear structure

描述（由申请人提供）：核被膜（NE）长期以来被认为是真核细胞基因组的看门人，其将基因组访问仅限制于能够经由核孔复合物（NPC）穿过NE的那些分子。然而，越来越明显的是，NE在基因组组织中发挥着积极和重要的作用，而不仅仅是提供简单的DMA区室化。NE相关复合物对于维持染色质结构、基因调控和DNA复制是重要的。NE结构的破坏可导致这些过程的失调。该建议侧重于NE膜蛋白在维持NE提供的结构完整性中的作用，特别关注NE膜蛋白的跨膜（TM）和内腔结构域的作用。

DESCRIPTION (provided by applicant): The nuclear envelope (NE) has long been appreciated as a gatekeeper to the genome of eukaryotic cells, restricting genomic access only to those molecules capable of traversing the NE via nuclear pore complexes (NPCs). It is increasingly evident, however, that the NE performs active and essential roles in genomic organization beyond providing simple compartmentalization of DMA. NE-associated complexes are important for maintenance of chromatin structure, gene regulation, and DMA replication. Disruption of the NE structure can lead to disregulation of these processes. This proposal focuses on the role of NE membrane proteins in maintaining the structural integrity provided by the NE, with a particular focus on the role of the transmembrane (TM) and lumenal domains of NE membrane proteins.