Characterization of novel core promoter elements

描述（由申请人提供）：本提案侧重于对核心推动者的理解。最近发现的核心启动子元件，motif ten element (MTE)，位于转录起始位点的下游。一项初步研究发现，MTE的转录需要一个（或多个）特定因子，而tata依赖性转录则不需要。这些因子可能是新的基础转录因子。本研究计划为系统研究MTE及其所需因素提供了一个大纲。此外，还建议开展一个项目，将CpG岛屿定性为核心启动子。具体而言，(1)纯化和表征介导mte依赖性转录的因子，这些因子可能是新的基础转录因子；(2)研究TFIID与MTE的相互作用；(3)研究CpG岛内具有核心启动子活性的序列。总之，这些研究将有助于理解核心启动子和基本的细胞过程-转录激活。

DESCRIPTION (provided by applicant): This proposal focuses on the understanding of core promoters. The most recent core promoter element identified, the motif ten element (MTE), is located downstream of the transcription start site. A preliminary study has found that transcription by the MTE requires a specific factor (or factors) that is not required for TATA-dependent transcription. These factors are likely to be novel basal transcription factors. This Research Proposal provides an outline for a systematic study of the MTE and its required factors. In addition, the initiation of a project involving the characterization of CpG islands as core promoters is proposed. Specifically, (1) to purify and characterize the factors that mediate MTE-dependent transcription, ones likely to be novel basal transcription factors; (2) investigate the interaction of TFIID with the MTE; (3) investigate the sequences within the CpG islands that have core promoter activity. Together, these studies will contribute to the understanding of core promoters and a fundamental cellular process- transcription activation.