Characterization of novel core promoter elements

描述（由申请人提供）：本提案侧重于对核心发起人的理解。最近鉴定的核心启动子元件，基序10元件（MTE），位于转录起始位点的下游。一项初步研究发现，MTE的转录需要一种特异性因子（或多种因子），而TATA依赖性转录不需要这种因子。这些因子可能是新的基础转录因子。本研究建议提供了一个大纲，系统研究的MTE及其所需的因素。此外，启动一个项目，涉及的CpG岛作为核心启动子的特性提出。具体而言，（1）纯化和表征介导MTE依赖性转录的因子，这些因子可能是新的基础转录因子;（2）研究TFIID与MTE的相互作用;（3）研究具有核心启动子活性的CpG岛内的序列。总之，这些研究将有助于理解核心启动子和一个基本的细胞过程-转录激活。

DESCRIPTION (provided by applicant): This proposal focuses on the understanding of core promoters. The most recent core promoter element identified, the motif ten element (MTE), is located downstream of the transcription start site. A preliminary study has found that transcription by the MTE requires a specific factor (or factors) that is not required for TATA-dependent transcription. These factors are likely to be novel basal transcription factors. This Research Proposal provides an outline for a systematic study of the MTE and its required factors. In addition, the initiation of a project involving the characterization of CpG islands as core promoters is proposed. Specifically, (1) to purify and characterize the factors that mediate MTE-dependent transcription, ones likely to be novel basal transcription factors; (2) investigate the interaction of TFIID with the MTE; (3) investigate the sequences within the CpG islands that have core promoter activity. Together, these studies will contribute to the understanding of core promoters and a fundamental cellular process- transcription activation.