Characterization of novel core promoter elements

新型核心启动子元件的表征

• 批准号: 7171853
• 负责人: Timur Yusufzai
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7171853
• 关键词: Affect; Be++ element; Beryllium; Binding; Cell physiology; Classification; CpG Islands; Elements; Gene Expression; General Transcription Factors; Genes; Genetic Transcription; Goals; Human; Indium; Mediating; Nucleotides; Proteins; RNA Polymerase II; Research Proposals; Role; Site; TATA Box; Testing; Transcription Factor TFIID; Transcription Initiation; Transcription Initiation Site; Transcriptional Activation; Transcriptional Regulation; Work; insight; novel; promoter

DESCRIPTION (provided by applicant): This proposal focuses on the understanding of core promoters. The most recent core promoter element identified, the motif ten element (MTE), is located downstream of the transcription start site. A preliminary study has found that transcription by the MTE requires a specific factor (or factors) that is not required for TATA-dependent transcription. These factors are likely to be novel basal transcription factors. This Research Proposal provides an outline for a systematic study of the MTE and its required factors. In addition, the initiation of a project involving the characterization of CpG islands as core promoters is proposed. Specifically, (1) to purify and characterize the factors that mediate MTE-dependent transcription, ones likely to be novel basal transcription factors; (2) investigate the interaction of TFIID with the MTE; (3) investigate the sequences within the CpG islands that have core promoter activity. Together, these studies will contribute to the understanding of core promoters and a fundamental cellular process- transcription activation.

说明(申请人提供)：本提案侧重于对核心发起人的理解。最近确定的核心启动子元件，基序10元件(MTE)，位于转录起始点的下游。一项初步研究发现，MTE的转录需要一种特定的因子，而TATA依赖的转录并不需要这种因子。这些因子很可能是新的基础转录因子。这份研究提案为系统研究MTE及其所需因素提供了一个概要。此外，还提议启动一个项目，将CpG岛定性为核心推动者。具体地说，(1)纯化和鉴定介导MTE依赖转录的因子，这些因子可能是新的基础转录因子；(2)研究TFIID与MTE的相互作用；(3)研究CpG岛内具有核心启动子活性的序列。总之，这些研究将有助于理解核心启动子和一个基本的细胞过程-转录激活。