Role of C elegans Nanos in germ cell fate

线虫 Nanos 在生殖细胞命运中的作用

• 批准号: 7054885
• 负责人: David J Katz
• 金额: $ 4.6万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7054885
• 关键词: Caenorhabditis elegans; RNA binding protein; RNA biosynthesis; RNA interference; cell differentiation; cell population study; developmental genetics; gene induction /repression; genetic screening; genetic translation; germ cells; invertebrate embryology; methyltransferase; postdoctoral investigator; transcription termination

DESCRIPTION (provided by applicant): As the germ line is the source of totipotant stem cells in most organisms, studies of how germ cells maintain totipotency are a wonderful model for understanding the maintenance of stem cell totipotency in general. Up until this point most studies of stem cell populations have focused on genes that must be expressed in stem cells to maintain their identity. However recently the germ lineage in many organisms has been shown to be transcriptionally quiescent, suggesting that the totipotency of these cells is largely maintained through repressive mechanisms. Therefore, understanding repressive mechanisms in the germ lineage is essential to understanding the mechanisms guarding stem cell identity. The gene Nanos is a translational repressor and has a role in maintaining the germ lineage from fly to human. The goal of this research is to understand the repressive mechanisms that are involved in stem cell maintenance by studying how the regulator Nanos maintains the germ line. Nanos function will be studied in C. elegans by ectopically expressing Nanos to determine which germ line characteristics it controls. In addition, both forward and reverse genetic approaches will be used to identify targets of Nanos translational repression.

描述（由申请人提供）：由于生殖系是大多数生物体中全能性干细胞的来源，研究生殖细胞如何保持全能性是理解干细胞全能性维持的一个很好的模型。到目前为止，对干细胞群体的大多数研究都集中在必须在干细胞中表达以保持其特性的基因上。然而，最近许多生物的生殖谱系已被证明是转录静止的，这表明这些细胞的全能性在很大程度上是通过抑制机制维持的。因此，了解生殖谱系中的抑制机制对于理解保护干细胞身份的机制至关重要。Nanos基因是一种翻译抑制因子，在维持从果蝇到人类的生殖谱系中起作用。本研究的目的是通过研究调节因子Nanos如何维持生殖系来了解参与干细胞维持的抑制机制。我们将通过异位表达Nanos来研究Nanos在秀丽隐杆线虫中的功能，以确定其控制的种系特征。此外，将使用正向和反向遗传方法来确定纳米翻译抑制的靶标。