Branched-chain fatty acids in C elegans development

线虫发育中的支链脂肪酸

• 批准号: 7102792
• 负责人: JENNIFER M BLANCHETTE
• 金额: $ 4.45万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7102792
• 关键词: Caenorhabditis elegans; RNA interference; alternatives to animals in research; bioenergetics; biological signal transduction; developmental genetics; fatty acids; gene environment interaction; genetic manipulation; growth /development; helminth genetics; insulin receptor; lipid biosynthesis; nutrition; nutrition related tag; phosphatidylinositols; phosphorylation; postdoctoral investigator; posttranslational modifications; protein structure; transcription factor

DESCRIPTION (provided by applicant): Our understanding of the role of lipid molecules in cell signaling has grown rapidly in recent years. Yet, we still do not have an understanding of the role of specific fatty acid constituents in affecting the signaling activities of lipids. Work in our laboratory has found that an unusual class of fatty acids, monomethyl branched-chain fatty acids (mmBCFAs), is essential for growth and development of the model organism C. elegans. C. elegans synthesize mmBCFAs de novo and in the absence of this biosynthetic pathway developmental arrest occurs. The experiments described in this proposal are aimed at understanding the essential roles of mmBCFAs in C. elegans growth and development by identifying gene products required to sense inadequate mmBCFA levels, investigating the genetic interactions of mmBCFAs with the daf-2/insulinlike receptor pathway and determining the activity of mmBCFAs on the CeTOR kinase pathway. It is hoped that these studies will enhance our understanding of why certain cells contain particular classes of fatty acids and how specific fatty acids may be modulating lipid signaling pathways.

描述（由申请人提供）：近年来，我们对脂质分子在细胞信号传导中的作用的理解迅速增长。但是，我们仍然不了解特定脂肪酸成分在影响脂质信号活性中的作用。我们实验室的工作发现，一类不寻常的脂肪酸，单甲基支链脂肪酸（MMBCFAS）对于模型有机体秀丽隐杆线虫的生长和发展至关重要。秀丽隐杆线虫合成了从头开始的MMBCFAS，并且在没有这种生物合成途径的情况下发生了发育停滞。该提案中描述的实验旨在通过鉴定MMBCFA水平不足所需的基因产品来理解MMBCFA在秀丽隐杆线虫的生长和发育中的重要作用，研究了MMBCFAS与DAF-2/胰岛素样受体途径的遗传相互作用，并确定MMMMMMMMCCFAS在cinase pathore的活性。希望这些研究能够增强我们对某些细胞包含特定类别的脂肪酸的理解，以及特定的脂肪酸如何调节脂质信号通路。