Effect of Leptin on Trophoblast Invasiveness

瘦素对滋养层细胞侵袭的影响

• 批准号: 7109294
• 负责人: Laura Clamon SCHULZ
• 金额: $ 5.04万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7109294
• 关键词: biological signal transduction; embryology; enzyme activity; epithelium; hormone regulation /control mechanism; laboratory mouse; leptin; metalloendopeptidases; microarray technology; mitogen activated protein kinase; nutrition; phosphorylation; placenta; postdoctoral investigator; preeclampsia; pregnancy; tissue /cell culture; trophoblast; uterus

DESCRIPTION (provided by applicant): I propose to investigate the relationship between leptin and trophobiast invasion. Invasion of the uterine epithelium by embryonic trophoblast ceils during early pregnancy is crucial for the establishment and development of the placenta, which is in turn necessary for healthy fetal development. Leptin is a hormone produced by adipose tissue, and in some species, the placenta, that acts as a signal of nutritional state to the reproductive system. The proposed project will attempt to characterize one of the mechanisms by which nutrition affects placental development, and increase our understanding of the role of leptin in pre-eclampsia, a common complication of pregnancy characterized by insufficient trophoblast invasion. The difference in invasivness between trophoblast ceils from early and late pregnancy will be used to study the effects of leptin. I will use primary mouse trophoblast ceils to test the hypotheses (1) that leptin promotes trophoblast invasion in placental ceils from early, but not late pregnancy and (2) that this invasion is mediated by an increase in MMP activity, which is (3) stimulated via the MAPK signaling pathway.

描述（由申请人提供）：我建议研究瘦素和练习式入侵之间的关系。怀孕初期胚胎滋养细胞天花板对子宫上皮的侵袭对于胎盘的建立和发展至关重要，这反过来又对健康的胎儿发育是必不可少的。瘦素是由脂肪组织产生的激素，在某些物种中，胎盘，它是生殖系统营养状态的信号。拟议的项目将试图表征营养影响胎盘发育的一种机制之一，并增强我们对瘦素在子痫前期中的作用的理解，这是妊娠的常见并发症，其特征是滋养细胞侵袭不足。妊娠早期和晚期的滋养细胞天花板之间的入侵性差异将用于研究瘦素的作用。我将使用原发性小鼠滋养细胞天花板来测试瘦素从早期促进胎盘天花板的滋养细胞侵袭的假设（1），但不会从早期妊娠中促进胎盘天花板，并且（2）（2）（2）（2）通过MMP活性的增加介导，这是（3）通过MAPK信号通路刺激的。

项目成果

Inhibition of trophoblast invasiveness in vitro by immunoneutralization of leptin in the bat, Myotis lucifugus (Chiroptera).

• DOI: 10.1016/j.ygcen.2006.07.007
• 发表时间: 2007
• 期刊: General and comparative endocrinology
• 影响因子: 2.7
• 作者: L. Schulz;K. Townsend;T. Kunz;E. Widmaier