Identification of Altered Glycan and Glycoproteins in Viral Induced Liver Cancer

病毒诱导的肝癌中改变的聚糖和糖蛋白的鉴定

• 批准号: 7198314
• 负责人: Anand S. Mehta
• 金额: $ 39.52万
• 依托单位: DREXEL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-26 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7198314
• 关键词: biomarker; glycoproteins; liver neoplasms; neoplasm /cancer; proteins; serum

DESCRIPTION (provided by applicant): This research grant will attempt to identify all the serum glycoproteins that have increased levels of fucosylation in the sera of patients with hepatocellular carcinoma (HCC). It is acknowledged in the literature that changes in glycosylation occur with cancer. More importantly we have shown that there is an increase in the level of core fucosylation associated with the development of liver cancer. However, the proteins on which these changes occur are, for the most part, unknown. Hence, we propose to evaluate serum, in both a pre-cancerous and cancerous state, for alterations in the amount of fucosylation associated with all serum glycoproteins. This comparative study will use a technology that will exploit a variety of fucose recognizing lectins to extract specific glycoproteins and identify them using LC MS/MS based proteomic methodologies. Glycoproteins that alter with the development of cancer will be evaluated further for their potential as biomarkers of virally induced liver cancer.

描述（由申请人提供）： 这项研究经费将试图确定所有的血清糖蛋白，增加了岩藻糖基化水平的肝细胞癌（HCC）患者的血清。在文献中承认糖基化的变化伴随癌症发生。更重要的是，我们已经表明，核心岩藻糖基化水平的增加与肝癌的发展有关。然而，发生这些变化的蛋白质在很大程度上是未知的。因此，我们建议评估血清，在癌前和癌状态下，与所有血清糖蛋白相关的岩藻糖基化量的变化。这项比较研究将使用一种技术，将利用各种岩藻糖识别凝集素提取特定的糖蛋白，并使用基于LC MS/MS的蛋白质组学方法鉴定它们。随着癌症的发展而改变的糖蛋白将进一步评估其作为病毒诱导的肝癌的生物标志物的潜力。