Identification of Altered Glycan and Glycoproteins in Viral Induced Liver Cancer

病毒诱导的肝癌中改变的聚糖和糖蛋白的鉴定

• 批准号: 7936016
• 负责人: Anand S. Mehta
• 金额: $ 30.61万
• 依托单位: DREXEL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7936016
• 关键词: Biological Assay; Biological Markers; Cancerous; Cohort Studies; Comparative Study; Detection; Development; Diagnosis; Disease; Fucose; Glycoproteins; Goals; Gold; Hepatitis B Virus; Human Papillomavirus; Learning; Lectin; Literature; Liver diseases; Malignant Neoplasms; Malignant neoplasm of cervix uteri; Malignant neoplasm of liver; Methodology; Methods; Mining; Patients; Polysaccharides; Predictive Value; Primary carcinoma of the liver cells; Proteins; Proteome; Proteomics; Research Project Grants; Sampling; Screening procedure; Sensitivity and Specificity; Serum; Serum Proteins; Technology; Testing; Ultrasonography; Validation; Viral; Work; base; cohort; comparative; glycosylation; tumor; validation studies

This research grant will attempt to identify all the serum glycoproteins that have increased levels of fucosylation in the sera of patients with hepatocellular carcinoma (HCC). It is acknowledged in the literature that changes in glycosylation occur with cancer. More importantly we have shown that there is an increase in the level of core fucosylation associated with the development of liver cancer. However, the proteins on which these changes occur are, for the most part, unknown. Hence, we propose to evaluate serum, in both a pre cancerous and cancerous state, for alterations in the amount of fucosylation associated with all serum glycoproteins. This comparative study will use a technology that will exploit a variety of fucose recognizing lectins to extract specific glycoproteins and identify them using LC MS/MS based proteomic methodologies. Glycoproteins that alter with the development of cancer will be evaluated further for their potential as biomarkers of virally induced liver cancer.

这项研究经费将试图确定所有的血清糖蛋白， 在患有肝细胞癌（HCC）的患者的血清中的岩藻糖基化。在文献中， 糖基化的改变会伴随着癌症的发生。更重要的是，我们已经表明， 核心岩藻糖基化水平与肝癌的发展相关。然而， 这些变化是如何发生的，在很大程度上是未知的。因此，我们建议评估血清，在这两个 癌前和癌状态，用于与所有血清相关的岩藻糖基化量的改变 糖蛋白这项比较研究将使用一种技术，将利用各种岩藻糖识别 凝集素提取特定的糖蛋白，并使用基于LC MS/MS的蛋白质组学方法鉴定它们。 随着癌症的发展而改变的糖蛋白将被进一步评估其潜力， 病毒诱导的肝癌的生物标志物。