High Speed MultiChannel Timing System for Imaging

用于成像的高速多通道计时系统

• 批准号: 7123440
• 负责人: John Graham White
• 金额: $ 6.55万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7123440
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; confocal scanning microscopy; fluorescence microscopy; fluorescent dye /probe; image processing; molecular /cellular imaging; photonics; spectrometry

DESCRIPTION (provided by applicant): Laser scanning, multiphoton fluorescence microscopy has become the method of choice for visualizing developmental and cellular dynamics of living tissue. Recently two new imaging modalities have been developed that provide extra dimensions of data from fluorescence signals. Spectral imaging can allow multiple fluorescent probes to be resolved even if their spectra significantly overlap. Lifetime imaging can provide information about the molecular environment of a probe and also has considerable potential for making accurate FRET measurements, a technique that can measure molecular interactions in vivo. We are proposing to develop a very high-speed time-correlated photon counting system that has 32 parallel channels. ft is designed to be used with a spectrometer that has been developed for multiphoton microscopy so t h a t simultaneous spectral and lifetime data can be acquired at photon counting rates that are more than an order ot magnitude greater that what can be obtained with currently-available instrumentation. The system uses ultra high-speed timer integrated circuits that have recently become available. The proposed system should be economical to produce and is being designed so that it can be easily added to currently available laser scanning microscopes.

描述(由申请人提供)： 激光扫描、多光子荧光显微镜已成为可视化活组织发育和细胞动力学的首选方法。最近已经开发了两种新的成像方式，它们可以从荧光信号中提供额外维度的数据。光谱成像可以使多个荧光探针得以分辨，即使它们的光谱显著重叠。寿命成像可以提供关于探针分子环境的信息，也有相当大的潜力进行精确的FRET测量，这是一种可以测量体内分子相互作用的技术。我们计划开发一种具有32个并行通道的超高速时间相关光子计数系统。FT是为多光子显微镜而开发的光谱仪，因此可以以比目前可用的仪器获得的大一个数量级以上的光子计数速率来获取同时的光谱和寿命数据。该系统使用了最近上市的超高速定时器集成电路。拟议的系统应该是经济的生产，并正在设计，以便它可以很容易地添加到目前可用的激光扫描显微镜。