Microscopy and Shared Equipment

显微镜和共享设备

• 批准号: 6915454
• 负责人: Frances M. Brodsky
• 金额: $ 5.52万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6915454
• 关键词: bioimaging /biomedical imaging; biomedical equipment resource; biomedical facility; cell biology; microscopy; natural killer cells; training; transfection

NK cell function depends on the balance between inhibitory and activating receptors expressed on the cell surface and how they are organized relative to each other in the NK cell-target cell synapse. For this reason a major goal of the program is to understand the cell biology of human NK cell function. Morphological studies proposed by all four units include localization of NK receptors and ligands in intracellular compartments and examination of their cell surface distribution at the NK cell-target cell synapse. In addition, membrane traffic pathways affecting receptor expression will be defined. All of these cell biological studies involve use of a deconvolution microscope facility to be provided by this Core. To understand the cell biology of individual NK receptors in a controlled context, many of the studies proposed will make use of transfectants produced by introduction of receptors into the NKL cell line or ligands into the 721.221 cell line. In order to produce such transfectants, this Core provides a shared AMAXA Nucleofector instrument. The Core also supports a shared Staff Research Associate (50% effort) who will be supervised by the Director, provide technical assistance for Core utilization and provide training in use of the Core facilities to personnel of the program. The specific aims of this Microscopy and Shared Equipment Core are to 1) support and maintain the deconvolution microscopy facility for the program and 2) purchase, support and maintain an AMAXA Nucleofector.

NK细胞的功能取决于细胞表面表达的抑制性和激活性受体之间的平衡，以及它们在NK细胞-靶细胞突触中的相对组织方式。出于这个原因，该计划的一个主要目标是了解人类NK细胞功能的细胞生物学。这四个单位提出的形态研究包括NK受体和配体在细胞内的定位，以及它们在NK细胞-靶细胞突触的细胞表面分布。此外，还将定义影响受体表达的膜交通通路。所有这些细胞生物学研究都涉及使用由本核心提供的去卷积显微镜设备。为了在受控的背景下了解单个NK受体的细胞生物学，许多研究将利用通过将受体导入NKL细胞系或将配体导入NKL细胞而产生的转染体。 721.221细胞系。为了生产这样的转染体，这个核心提供了一个共享的AMAXA核探测器仪器。核心还支持一名共享工作人员研究助理(50%的工作)，该助理将由主任监督，为核心设施的使用提供技术援助，并为方案人员提供使用核心设施的培训。该显微镜和共享设备核心的具体目标是：1)支持和维护用于该计划的反卷积显微镜设备；2)购买、支持和维护AMAXA核探测器。