Microscopy and Shared Equipment

显微镜和共享设备

• 批准号: 6915454
• 负责人: Frances M. Brodsky
• 金额: $ 5.52万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6915454
• 关键词: bioimaging /biomedical imaging; biomedical equipment resource; biomedical facility; cell biology; microscopy; natural killer cells; training; transfection

NK cell function depends on the balance between inhibitory and activating receptors expressed on the cell surface and how they are organized relative to each other in the NK cell-target cell synapse. For this reason a major goal of the program is to understand the cell biology of human NK cell function. Morphological studies proposed by all four units include localization of NK receptors and ligands in intracellular compartments and examination of their cell surface distribution at the NK cell-target cell synapse. In addition, membrane traffic pathways affecting receptor expression will be defined. All of these cell biological studies involve use of a deconvolution microscope facility to be provided by this Core. To understand the cell biology of individual NK receptors in a controlled context, many of the studies proposed will make use of transfectants produced by introduction of receptors into the NKL cell line or ligands into the 721.221 cell line. In order to produce such transfectants, this Core provides a shared AMAXA Nucleofector instrument. The Core also supports a shared Staff Research Associate (50% effort) who will be supervised by the Director, provide technical assistance for Core utilization and provide training in use of the Core facilities to personnel of the program. The specific aims of this Microscopy and Shared Equipment Core are to 1) support and maintain the deconvolution microscopy facility for the program and 2) purchase, support and maintain an AMAXA Nucleofector.

NK细胞功能取决于细胞表面上表达的抑制性和活化性受体之间的平衡，以及它们在NK细胞-靶细胞突触中相对于彼此如何组织。出于这个原因，该计划的一个主要目标是了解人类NK细胞功能的细胞生物学。所有四个单位提出的形态学研究包括NK受体和配体在细胞内室的定位和检查它们在NK细胞-靶细胞突触的细胞表面分布。此外，将定义影响受体表达的膜交通途径。所有这些细胞生物学研究都涉及使用该核心提供的去卷积显微镜设施。为了理解在受控背景下单个NK受体的细胞生物学，提出的许多研究将利用通过将受体引入NKL细胞系或将配体引入NKL细胞系而产生的转染子。 721.221细胞系。为了产生这样的转染子，该核心提供了共享的AMAXA Nucleofector仪器。核心还支持共享的工作人员研究助理（50%的努力）谁将由主任监督，提供核心利用技术援助，并提供培训，在使用核心设施的程序的人员。该显微镜和共享设备核心的具体目标是：1）支持和维护该计划的去卷积显微镜设施; 2）购买、支持和维护AMAXA Nucleofector。